Long Non-Coding RNA GAS5 and Intestinal MMP2 and MMP9 Expression: A Translational Study in Pediatric Patients with IBD.

BACKGROUND

The long non-coding RNA (lncRNA) growth arrest-specific transcript 5 () seems to be involved in the regulation of mediators of tissue injury, in particular matrix metalloproteinases (MMPs), implicated in the pathogenesis of inflammatory bowel disease (IBD). We investigated the role of in regulating and expression in pediatric patients with IBD and in vitro.

METHODS

In total, 25 IBD patients were enrolled: For each patient paired inflamed and non-inflamed biopsies were collected. RNA was extracted and , , and were quantified by TaqMan assay. The expression of and MMPs was also determined in the human monocytic THP1 cells differentiated into macrophages and stimulated with lipopolysaccharide (LPS). The function of was assessed by overexpressing the lncRNA and evaluating the MMPs levels.

RESULTS

Real-time PCR results demonstrated a downregulation of and an upregulation of both MMPs in inflamed tissues. In vitro data confirmed the trend observed in patients for the three genes: The stimulation with LPS promoted a downregulation of while an increase of MMPs was observed. Overexpression experiments showed that higher levels of lead to a decrease of both enzymes.

CONCLUSION

These results provide new information about the role of in IBD: The lncRNA could mediate tissue damage by modulating the expression of MMPs.