Burgess Helen J, Park Margaret, Wyatt James K, Fogg Louis F
Biological Rhythms Research Laboratory, Rush University Medical Center, Chicago, IL, USA.
Sleep Disorders Service and Research Center, Rush University Medical Center, Chicago, IL, USA.
J Sleep Res. 2016 Jun;25(3):314-7. doi: 10.1111/jsr.12384. Epub 2016 Feb 5.
The dim light melatonin onset (DLMO) assists with the diagnosis and treatment of circadian rhythm sleep disorders. Home DLMOs are attractive for cost savings and convenience, but can be confounded by home lighting and sample timing errors. We developed a home saliva collection kit with objective measures of light exposure and sample timing. We report on our first test of the kit in a clinical population. Thirty-two participants with delayed sleep phase disorder (DSPD; 17 women, aged 18-52 years) participated in two back-to-back home and laboratory phase assessments. Most participants (66%) received at least one 30-s epoch of light >50 lux during the home phase assessments, but for only 1.5% of the time. Most participants (56%) collected every saliva sample within 5 min of the scheduled time. Eighty-three per cent of home DLMOs were not affected by light or sampling errors. The home DLMOs occurred, on average, 10.2 min before the laboratory DLMOs, and were correlated highly with the laboratory DLMOs (r = 0.93, P < 0.001). These results indicate that home saliva sampling with objective measures of light exposure and sample timing, can assist in identifying accurate home DLMOs.
暗光褪黑素起始时间（DLMO）有助于昼夜节律性睡眠障碍的诊断和治疗。家庭DLMO测量因其节省成本和方便而具有吸引力，但可能会受到家庭照明和样本采集时间误差的干扰。我们开发了一种家庭唾液采集试剂盒，可客观测量光照暴露和样本采集时间。我们报告了该试剂盒在临床人群中的首次测试情况。32名患有睡眠相位延迟障碍（DSPD；17名女性，年龄18 - 52岁）的参与者参加了连续两次家庭和实验室阶段评估。在家庭阶段评估期间，大多数参与者（66%）至少有一次30秒的光照强度>50勒克斯，但仅占总时间的1.5%。大多数参与者（56%）在预定时间的5分钟内采集了每一份唾液样本。83%的家庭DLMO测量结果未受光照或采样误差的影响。家庭DLMO平均比实验室DLMO提前10.2分钟出现，并且与实验室DLMO高度相关（r = 0.93，P < 0.001）。这些结果表明，采用客观测量光照暴露和样本采集时间的家庭唾液采样，有助于确定准确的家庭DLMO。
