Burgess Helen J, Wyatt James K, Park Margaret, Fogg Louis F
Biological Rhythms Research Laboratory, Department of Behavioral Sciences, Rush University Medical Center, Chicago, IL.
Sleep Disorders Service and Research Center, Department of Behavioral Sciences, Rush University Medical Center, Chicago, IL.
Sleep. 2015 Jun 1;38(6):889-97. doi: 10.5665/sleep.4734.
There is a need for the accurate assessment of circadian phase outside of the clinic/laboratory, particularly with the gold standard dim light melatonin onset (DLMO). We tested a novel kit designed to assist in saliva sampling at home for later determination of the DLMO. The home kit includes objective measures of compliance to the requirements for dim light and half-hourly saliva sampling.
Participants were randomized to one of two 10-day protocols. Each protocol consisted of two back-to-back home and laboratory phase assessments in counterbalanced order, separated by a 5-day break.
Laboratory or participants' homes.
Thirty-five healthy adults, age 21-62 y.
N/A.
Most participants received at least one 30-sec epoch of light > 50 lux during the home phase assessments (average light intensity 4.5 lux), but on average for < 9 min of the required 8.5 h. Most participants collected every saliva sample within 5 min of the scheduled time. Ninety-two percent of home DLMOs were not affected by light > 50 lux or sampling errors. There was no significant difference between the home and laboratory DLMOs (P > 0.05); on average the home DLMOs occurred 9.6 min before the laboratory DLMOs. The home DLMOs were highly correlated with the laboratory DLMOs (r = 0.91, P < 0.001).
Participants were reasonably compliant to the home phase assessment procedures. The good agreement between the home and laboratory dim light melatonin onsets (DLMOs) demonstrates that including objective measures of light exposure and sample timing during home saliva sampling can lead to accurate home DLMOs.
Circadian Phase Assessments at Home, http://clinicaltrials.gov/show/NCT01487252, NCT01487252.
需要在诊所/实验室之外准确评估昼夜节律相位，尤其是采用金标准暗光褪黑素起始时间（DLMO）。我们测试了一种新型试剂盒，该试剂盒旨在辅助在家中采集唾液，以便日后测定DLMO。家用试剂盒包括对暗光要求和半小时一次唾液采样要求的依从性的客观测量。
参与者被随机分配到两个为期10天的方案之一。每个方案包括两个背靠背的家庭和实验室阶段评估，评估顺序平衡，中间间隔5天。
实验室或参与者家中。
35名年龄在21 - 62岁的健康成年人。
无。
在家庭阶段评估期间，大多数参与者至少有一次30秒的光照超过50勒克斯（平均光照强度4.5勒克斯），但在所需的8.5小时内平均光照时间不到9分钟。大多数参与者在预定时间的5分钟内采集了每一份唾液样本。92%的家庭DLMO不受超过50勒克斯的光照或采样误差影响。家庭和实验室的DLMO之间没有显著差异（P>0.05）；平均而言，家庭DLMO比实验室DLMO早出现9.6分钟。家庭DLMO与实验室DLMO高度相关（r = 0.91，P < 0.001）。
参与者对家庭阶段评估程序的依从性较好。家庭和实验室暗光褪黑素起始时间（DLMO）之间的良好一致性表明，在家庭唾液采样期间纳入光照暴露和采样时间的客观测量可以得出准确的家庭DLMO。
在家进行昼夜节律相位评估，http://clinicaltrials.gov/show/NCT01487252，NCT01487252。
