Li Xiaofang, Zang Aimin, Jia Youchao, Zhang Jinchao, Fan Wufang, Feng Jia, Duan Mindan, Zhang Lei, Huo Ran, Jiao Jin, Zhu Xiaowei
Hebei Key Laboratory of Cancer Radiotherapy and Chemotherapy, Department of Medical Oncology, Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China.
College of Chemistry and Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding, Hebei 071002, P.R. China.
Mol Med Rep. 2016 Mar;13(3):2763-8. doi: 10.3892/mmr.2016.4844. Epub 2016 Feb 2.
Triptolide is used in traditional Chinese medicine. It has the advantages of a unique mechanism of action, a wide antitumor spectrum, multiple targets, multi-channel effects and low toxicity. The current study was conducted to evaluate whether the potential anticancer effects of triptolide reduces proliferation and enhances apoptosis of human non‑small cell lung cancer (NSCLC) cells, and to assess the underlying anticancer mechanisms. In PC‑9 cells, treatment with triptolide reduced cell proliferation and increased cell apoptosis and caspase‑3 and 9 activity. Triptolide treatment reduced miR‑21 expression and enhanced phosphatase and tensin homolog (PTEN) protein expression levels in the PC‑9 cells. Furthermore, the upregulation of miR‑21 expression levels suppressed the effect of triptolide on cell viability and PTEN protein expression levels in PC‑9 cells. To the best of our knowledge, the present study is the first to demonstrate that triptolide reduced the proliferation and enhanced the apoptosis of human NSCLC cells through PTEN by targeting miR-21.
雷公藤甲素用于传统中药。它具有作用机制独特、抗肿瘤谱广、多靶点、多途径作用和低毒性等优点。本研究旨在评估雷公藤甲素潜在的抗癌作用是否能降低人非小细胞肺癌（NSCLC）细胞的增殖并增强其凋亡，并评估其潜在的抗癌机制。在PC-9细胞中，雷公藤甲素处理可降低细胞增殖，增加细胞凋亡以及半胱天冬酶-3和-9的活性。雷公藤甲素处理降低了PC-9细胞中miR-21的表达，并提高了磷酸酶和张力蛋白同源物（PTEN）蛋白的表达水平。此外，miR-21表达水平的上调抑制了雷公藤甲素对PC-9细胞活力和PTEN蛋白表达水平的影响。据我们所知，本研究首次证明雷公藤甲素通过靶向miR-21，经由PTEN降低人NSCLC细胞的增殖并增强其凋亡。
