长链非编码RNA lncRNA-ATB的高表达与转移相关，并促进肾细胞癌的细胞迁移和侵袭。
High expression of long non-coding RNA lncRNA-ATB is correlated with metastases and promotes cell migration and invasion in renal cell carcinoma.
作者信息
Xiong Jing, Liu Ying, Jiang Li, Zeng Yang, Tang Wei
机构信息
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Department of Urology and Andrology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei Province, China.
Department of Preventive Medicine, School of Public Administration, Hubei University of Medicine, Shiyan, Hubei Province, China.
出版信息
Jpn J Clin Oncol. 2016 Apr;46(4):378-84. doi: 10.1093/jjco/hyv214. Epub 2016 Feb 3.
OBJECTIVE
To investigate the expression pattern of a novel long non-coding ribonucleic acid activated by transforming growth factor β, long non-coding ribonucleic acid activated by transforming growth factor β, in renal cell carcinoma tissues among the patients with various clinicopathologic features and to detect the possible role of dysregulated long non-coding RNA-ATB in renal cell carcinoma.
METHODS
The expression of long non-coding ribonucleic acid activated by transforming growth factor β in the renal cell carcinoma tissues and renal cancer cell lines was evaluated by quantitative real-time polymerase chain reaction. The association with clinicopathologic features was analyzed. The effects of long non-coding ribonucleic acid activated by transforming growth factor β on the renal cell carcinoma cell proliferation, apoptosis, migration, invasion and epithelial-to-mesenchymal transition were investigated by the loss-of-function approach.
RESULTS
The expression of long non-coding ribonucleic acid activated by transforming growth factor β was higher in the renal cell carcinoma tissues and renal cancer cells than in the adjacent non-tumor tissues and normal human proximal tubule epithelial cells HK-2. In addition, the long non-coding ribonucleic acid activated by transforming growth factor β expression was significantly higher in the renal cell carcinoma patients with metastasis. The elevated expression of long non-coding ribonucleic acid activated by transforming growth factor β was associated with tumor stages, histological grade, vascular invasion, lymph node metastasis and distant metastasis. Notably, we found that long non-coding ribonucleic acid activated by transforming growth factor β knockdown could (i) inhibit cell proliferation; (ii) trigger apoptosis; (iii) reduce epithelial-to-mesenchymal transition program; (iv) suppress cell migration and invasion.
CONCLUSIONS
Our data have shown that long non-coding ribonucleic acid activated by transforming growth factor β actively functions as a regulator of epithelial-to-mesenchymal transition during renal cell carcinoma metastasis, which suggests that long non-coding ribonucleic acid activated by transforming growth factor β may be a potential prognostic biomarker and therapeutic target for renal cell carcinoma.
目的
研究一种由转化生长因子β激活的新型长链非编码核糖核酸（lncRNA），即转化生长因子β激活的长链非编码核糖核酸（lncRNA-ATB），在不同临床病理特征的肾细胞癌组织中的表达模式，并检测失调的lncRNA-ATB在肾细胞癌中的可能作用。
方法
采用定量实时聚合酶链反应评估转化生长因子β激活的长链非编码核糖核酸在肾细胞癌组织和肾癌细胞系中的表达。分析其与临床病理特征的相关性。通过功能缺失方法研究转化生长因子β激活的长链非编码核糖核酸对肾癌细胞增殖、凋亡、迁移、侵袭及上皮-间质转化的影响。
结果
转化生长因子β激活的长链非编码核糖核酸在肾细胞癌组织和肾癌细胞中的表达高于相邻非肿瘤组织和正常人近端肾小管上皮细胞HK-2。此外，在有转移的肾细胞癌患者中，转化生长因子β激活的长链非编码核糖核酸表达明显更高。转化生长因子β激活的长链非编码核糖核酸表达升高与肿瘤分期、组织学分级、血管侵犯、淋巴结转移和远处转移相关。值得注意的是，我们发现敲低转化生长因子β激活的长链非编码核糖核酸可（i）抑制细胞增殖；（ii）诱导凋亡；（iii）减少上皮-间质转化程序；（iv）抑制细胞迁移和侵袭。
结论
我们的数据表明，转化生长因子β激活的长链非编码核糖核酸在肾细胞癌转移过程中作为上皮-间质转化的调节因子发挥积极作用，这表明转化生长因子β激活的长链非编码核糖核酸可能是肾细胞癌潜在的预后生物标志物和治疗靶点。
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