First Affiliated Hospital of Dalian Medical University, Dalian, China.
Dalian Municipal Central Hospital Affiliated of Dalian Medical University, Dalian, China.
J Mater Sci Mater Med. 2019 Apr 29;30(5):53. doi: 10.1007/s10856-019-6257-3.
Poly (glycerol sebacate) (PGS) is a synthetic polymeric material with the characteristics of controllable degradation, high plasticity and excellent biocompatibility. However, the time of PGS degradation is faster than that of cartilage regeneration, which limits its application in cartilage tissue engineering. Polycaprolactone (PCL), a widely used synthetic polymer, has appropriate biodegradability and higher mechanical strength. This study aims to make a scaffold from blends of fast degrading PGS and slowly degrading PCL, and to investigate its potential for cartilage tissue engineering applications. Scanning electron microscopic analysis indicated that the scaffolds provided favourable porous microstructures. In vitro degradation test showed that PGS/ PCL scaffolds acquired longer degradation time and better mechanical strength. PGS/PCL scaffolds seeded with Bone marrow-derived mesenchymal stem cells (BMSCs) and articular chondrocytes (ACCs) were cultured in vitro. Short-term in vitro experiments confirmed that both seeded cells could adhere and proliferate on the scaffold. Chondrogenic culture for cell-scaffold constructs confirmed BMSCs could differentiate into chondrocyte-like cells in PGS/PCL scaffolds. With tunable biodegradation, favorable mechanical properties and cytocompatibility, PGS/PCL scaffolds would potentially be suitable for the regeneration of cartilage tissue. Poly (glycerol sebacate) (PGS) is a synthetic polymeric material with the characteristics of controllable degradation, high plasticity and good biocompatibility. However, the time of PGS degradation is faster than that of cartilage regeneration, which limits its application in cartilage tissue engineering. Polycaprolactone(PCL), a widely used synthetic polymer, has appropriate biodegradability. This study aims to make a scaffold from blends of fast degrading PGS and slowly degrading PCL, and to investigate its potential for cartilage tissue engineering applications. Scanning electron microscopic analysis indicated that the scaffolds provided favourable porous microstructures. In vitro degradation test showed that PGS/ PCL scaffolds got longer degradation time with surface degradation nature. PGS/PCL scaffolds seeded with Bone marrow-derived mesenchymal stem cells (BMSCs) and articular chondrocytes (ACCs) were cultured in vitro under the same condition. Short-term in vitro experiments confirmed that both seed cells could adhere and proliferate on the scaffold. Chondrogenic culture for cell-scaffold constructs confirmed BMSCs could differentiate into chondrocyte-like cells and form cartilage-specific matrix in PGS/PCL scaffolds. With cytocompatibility and biodegradation profile, PGS/PCL scaffolds get great potential for cartilage tissue engineering.
聚（癸二酸丙二醇酯）（PGS）是一种具有可控降解性、高可塑性和良好生物相容性的合成聚合物材料。然而，PGS 的降解时间快于软骨再生，这限制了它在软骨组织工程中的应用。聚己内酯（PCL）是一种广泛使用的合成聚合物，具有适当的生物降解性和更高的机械强度。本研究旨在制备由快速降解的 PGS 和缓慢降解的 PCL 组成的支架，并研究其在软骨组织工程中的应用潜力。扫描电子显微镜分析表明，支架具有良好的多孔微观结构。体外降解试验表明，PGS/PCL 支架的降解时间延长，机械强度提高。骨髓间充质干细胞（BMSCs）和关节软骨细胞（ACCs）接种到 PGS/PCL 支架上，在体外进行培养。短期体外实验证实，两种接种细胞均可在支架上黏附和增殖。对细胞-支架构建体进行软骨形成培养证实，BMSCs 可在 PGS/PCL 支架中分化为软骨细胞样细胞。PGS/PCL 支架具有可调节的生物降解性、良好的机械性能和细胞相容性，有望适用于软骨组织的再生。
