Li Tao-Tao, Li Xiao-Yan, Jia Li-Xin, Zhang Jing, Zhang Wen-Mei, Li Yu-Lin, Qi Yong-Fen, Du Jie
Beijing Anzhen Hospital, Capital Medical University; The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education; Beijing Collaborative Innovation Center for Cardiovascular Disorders; Beijing Institute of Heart, Lung & Blood Vessel Disease, Beijing, China.
Cell Physiol Biochem. 2016;38(2):670-82. doi: 10.1159/000438659. Epub 2016 Feb 8.
BACKGROUND/AIMS: Hypertension plays a critical role in the cardiac inflammation and injury. However, the mechanism of how hypertension causes the cardiac injury at a molecular level remains to be elucidated.
RNA-Seq has been demonstrated to be an effective approach for transcriptome analysis, which is essential to reveal the molecular constituents of cells and tissues. In this study, we investigated the global molecular events associated with the mechanism of hypertension induced cardiac injury using RNA-Seq analysis.
Our results showed that totally 1,801 genes with different expression variations were identified after Ang II infusion at 1, 3 and 7 days. Go analysis showed that the top 5 high enrichment Go terms were response to stress, response to wounding, cellular component organization, cell activation and defense response. KEGG pathway analysis revealed the top 5 significantly overrepresented pathways were associated with ECM-receptor interaction, focal adhesion, protein digestion and absorption, phagosome and asthma. Moreover, protein-protein interaction network analysis indicated that ubiquitin C may play a key role in the processes of hypertension-induced cardiac injury.
Our study provides a comprehensive understanding of the transcriptome events in hypertension-induced cardiac pathology.
背景/目的：高血压在心脏炎症和损伤中起关键作用。然而，高血压在分子水平上导致心脏损伤的机制仍有待阐明。
RNA测序已被证明是一种用于转录组分析的有效方法，这对于揭示细胞和组织的分子组成至关重要。在本研究中，我们使用RNA测序分析研究了与高血压诱导的心脏损伤机制相关的整体分子事件。
我们的结果表明，在第1、3和7天输注血管紧张素II后，共鉴定出1801个具有不同表达变化的基因。基因本体（Go）分析显示，前5个高富集的Go术语是对应激的反应、对损伤的反应、细胞成分组织、细胞活化和防御反应。京都基因与基因组百科全书（KEGG）通路分析显示，前5个显著富集的通路与细胞外基质-受体相互作用、粘着斑、蛋白质消化和吸收、吞噬体和哮喘有关。此外，蛋白质-蛋白质相互作用网络分析表明，泛素C可能在高血压诱导的心脏损伤过程中起关键作用。
我们的研究提供了对高血压诱导的心脏病理中转录组事件的全面理解。
