Tolle Fabian, Rosenthal Malte, Pfeiffer Franziska, Mayer Günter
Life and Medical Sciences Institute, University of Bonn , Gerhard-Domagk-Str. 1, 53121 Bonn, Germany.
Bioconjug Chem. 2016 Mar 16;27(3):500-3. doi: 10.1021/acs.bioconjchem.5b00668. Epub 2016 Feb 11.
The post-synthetic functionalization of nucleic acids via click chemistry (CuAAC) has seen tremendous implementation, extending the applicability of nucleobase-modified nucleic acids in fields like fluorescent labeling, nanotechnology, and in vitro selection. However, the production of large quantities of high-density functionalized material via solid phase synthesis has been hampered by oxidative by-product formation associated with the alkaline workup conditions. Herein, we describe a rapid and cost-effective protocol for the high fidelity large-scale production of nucleobase-modified nucleic acids, exemplified with a recently described nucleobase-modified aptamer.
通过点击化学（CuAAC）对核酸进行合成后功能化已得到广泛应用，扩展了核碱基修饰核酸在荧光标记、纳米技术和体外筛选等领域的适用性。然而，通过固相合成大量生产高密度功能化材料受到与碱性后处理条件相关的氧化副产物形成的阻碍。在此，我们描述了一种用于高保真大规模生产核碱基修饰核酸的快速且经济高效的方案，并以最近描述的核碱基修饰适体为例进行说明。
