采用被动血凝试验检测全血和唾液中抗1型人类免疫缺陷病毒抗体。

Detection of antibodies to human immunodeficiency virus type 1 in whole blood and saliva by using a passive hemagglutination test.

作者信息

Vasudevachari M B, Uffelman K, Pierce P F, Lane H C, Salzman N P

机构信息

Department of Microbiology, Georgetown University School of Medicine, Washington, D.C. 20007.

出版信息

J Clin Microbiol. 1989 Oct;27(10):2384-5. doi: 10.1128/jcm.27.10.2384-2385.1989.

DOI:10.1128/jcm.27.10.2384-2385.1989

PMID:2685034

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC267031/

Abstract

A passive hemagglutination test (PHA) for detecting human immunodeficiency virus type 1 antibodies in serum samples by using envelope glycoprotein (gp160)-coupled sheep erythrocytes was described earlier (M.B. Vasudevachari, K. U. Uffelman, T.C. Mast, R.L. Dewar, V. Natarajan, H.C. Lane, and N.P. Salzman, J. Clin. Microbiol. 27:179-181, 1989). In the study reported here, the applicability of the PHA test to the detection of antibodies in whole-blood and saliva samples has been investigated. We observed a 100% correlation between PHA and commercial enzyme-linked immunosorbent assay in 101 whole-blood samples and 98% correlation between PHA and reactivity to envelope proteins in Western blots (immunoblots) of 53 saliva samples. Furthermore, salivary antibodies could be detected in 19 of the 22 seropositive individuals. As in serum, antibodies to envelope proteins were widely prevalent in all the Western blot-reactive saliva samples.

摘要

先前已描述了一种通过使用包膜糖蛋白（gp160）偶联的绵羊红细胞来检测血清样本中1型人类免疫缺陷病毒抗体的被动血凝试验（PHA）（M.B. Vasudevachari、K.U. Uffelman、T.C. Mast、R.L. Dewar、V. Natarajan、H.C. Lane和N.P. Salzman，《临床微生物学杂志》27:179 - 181，1989年）。在本文报道的研究中，已对PHA试验在全血和唾液样本中检测抗体的适用性进行了研究。我们观察到，在101份全血样本中，PHA与商业酶联免疫吸附测定之间的相关性为100%，在53份唾液样本的蛋白质免疫印迹（免疫印迹）中，PHA与包膜蛋白反应性之间的相关性为98%。此外，在22名血清阳性个体中的19名中检测到了唾液抗体。与血清中一样，包膜蛋白抗体在所有蛋白质免疫印迹反应性唾液样本中广泛存在