Parry J V, Perry K R, Mortimer P P
Lancet. 1987 Jul 11;2(8550):72-5. doi: 10.1016/s0140-6736(87)92737-1.
Paired serum and saliva specimens were tested by conventional assays and by IgG-capture radioimmunoassays (GACRIA) and enzyme-linked immunosorbent assays (GACELISA) for antibody to hepatitis A virus (HAV, 100 pairs), human immunodeficiency virus (HIV, 53), hepatitis B virus core (HBc, 62), and rubella virus (30). Conventional assays failed to detect viral antibodies in the saliva of 93 of 119 seropositive subjects. However, GACRIA detected the antibodies in both serum and saliva of all subjects seropositive by conventional tests, except 2 saliva specimens false-negative for anti-HBc and 1 false-negative for anti-rubella-virus. For anti-HIV and anti-HBc serum and saliva GACRIA reactivities did not differ significantly, but anti-HAV and anti-rubella-virus GACRIA reactivities were stronger in serum than saliva. GACRIA and GACELISA results on saliva for the four antibodies correlated closely; for anti-HAV and anti-HIV GACELISA and GACRIA were equally accurate. For both saliva and serum, GACRIA was superior to an IgA-capture assay in detecting anti-HAV and anti-HIV.
采用传统检测方法以及IgG捕获放射免疫分析（GACRIA）和酶联免疫吸附分析（GACELISA）对配对的血清和唾液样本进行检测，以检测甲型肝炎病毒（HAV，100对）、人类免疫缺陷病毒（HIV，53对）、乙型肝炎病毒核心抗体（HBc，62对）和风疹病毒（30对）的抗体。传统检测方法未能在119名血清学阳性受试者中的93人的唾液中检测到病毒抗体。然而，GACRIA在所有经传统检测血清学阳性的受试者的血清和唾液中均检测到了抗体，但有2份抗-HBc唾液样本呈假阴性，1份抗风疹病毒唾液样本呈假阴性。对于抗-HIV和抗-HBc，血清和唾液的GACRIA反应性无显著差异，但抗-HAV和抗风疹病毒的GACRIA反应性在血清中比在唾液中更强。四种抗体在唾液中的GACRIA和GACELISA结果密切相关；对于抗-HAV和抗-HIV，GACELISA和GACRIA同样准确。对于唾液和血清，在检测抗-HAV和抗-HIV方面，GACRIA优于IgA捕获分析。
