Sun D, Archibald D W, Furth P A
Department of Oral Pathology, University of Maryland, Baltimore 21201.
AIDS Res Hum Retroviruses. 1990 Jul;6(7):933-41. doi: 10.1089/aid.1990.6.933.
The secretory immune response to pathogens of the gut-associated lymphoid tissue is often independent of the systemic response. We investigated and compared the presence of antibodies to human immunodeficiency virus type 1 (HIV-1) antigens in parotid saliva and serum by Western blotting in 22 HIV-1-infected individuals. Antibodies to the HIV-1 envelope antigen gp160 were detected in saliva samples from 21 of 22 individuals and in serum from all individuals who were classified as CDC Group II, III, or IV. Antibody titers to gp160 were approximately 3000 times higher in serum than in saliva. Antibodies to viral core antigen p24 were detected in 6 of 7 Group II individuals in saliva and in 7 of 7 in serum. Antibodies to p24 were not found in the parotid saliva, but were detected in the sera of 3 of 3 Group III and 11 of 12 Group IV patients. The absence of secretory antibodies to HIV-1 core antigen p24 was correlated with CD4+ cell counts of less than 200/mm3. The results suggest that loss of secretory anti-p24 antibodies may be an early sign of progression to higher CDC clinical stages in HIV-1-infected individuals.
肠道相关淋巴组织对病原体的分泌性免疫反应通常独立于全身反应。我们通过蛋白质印迹法对22名感染人类免疫缺陷病毒1型（HIV-1）的个体的腮腺唾液和血清中抗HIV-1抗原的抗体进行了检测和比较。在22名个体中的21名的唾液样本以及所有被分类为疾病控制与预防中心（CDC）II、III或IV组的个体的血清中检测到了抗HIV-1包膜抗原gp160的抗体。血清中针对gp160的抗体滴度比唾液中的高约3000倍。在7名II组个体中的6名唾液以及7名个体中的7名血清中检测到了针对病毒核心抗原p24的抗体。在腮腺唾液中未发现针对p24的抗体，但在3名III组患者中的3名以及12名IV组患者中的11名的血清中检测到了该抗体。缺乏针对HIV-1核心抗原p24的分泌性抗体与CD4 +细胞计数低于200/mm3相关。结果表明，分泌性抗p24抗体的丧失可能是HIV-1感染个体进展到更高CDC临床阶段的早期迹象。
