DU Qiao-Hui, Zhang Qiao-Yan, Han Ting, Jiang Yi-Ping, Peng Cheng, Xin Hai-Liang
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China; Key Laboratory of Standardization of Chinese Herbal Medicines of the Ministry of Education, College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610000, China.
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Chin J Nat Med. 2016 Jan;14(1):66-72. doi: 10.3724/SP.J.1009.2015.00066.
Flavonoids are a large group of phenolic secondary metabolites havinga wide range of biochemical and pharmacological effects. Quantitative analysis of flavonoid profiles in the genus Actinidia, which has not been intensively conducted, is useful to a better understanding of the pattern and distribution of flavonoids. In the present work, a liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method was developed to profile the flavonoids, which was then used to determine the dynamic change of 17 biologically active flavonoids in the leaves of Actinidia valvata at the main growing stages, including glucuronides and acylated di- and triglycosides of flavonoids. The contents of flavonoid triglycosides were significantly higher than other flavonoids. The highest concentrations of kaemperol glycosides were observed in June, while other flavonoids showed highest concentrations in October. On the other hand, the contents of four isorhamnetin glycosides were increased sharply in September to October. The flavonoid profiles seem to be related to temperature, UV-B, and water deficit. Further studies are required to examine the functions of flavonoids in the Actinidia valvata and the underlying molecular mechanisms of actions.
黄酮类化合物是一大类具有广泛生化和药理作用的酚类次生代谢产物。对猕猴桃属中黄酮类化合物谱的定量分析尚未深入开展，这对于更好地理解黄酮类化合物的模式和分布很有帮助。在本研究中，开发了一种液相色谱 - 电喷雾电离 - 串联质谱（LC - ESI - MS/MS）方法来分析黄酮类化合物，然后用该方法测定了中华猕猴桃叶片在主要生长阶段17种生物活性黄酮类化合物的动态变化，包括黄酮类化合物的葡萄糖醛酸苷以及酰化二糖苷和三糖苷。黄酮类三糖苷的含量显著高于其他黄酮类化合物。山奈酚苷的最高浓度在6月观察到，而其他黄酮类化合物在10月浓度最高。另一方面，四种异鼠李素苷的含量在9月至10月急剧增加。黄酮类化合物谱似乎与温度、UV - B和水分亏缺有关。需要进一步研究来考察黄酮类化合物在中华猕猴桃中的功能及其潜在的分子作用机制。
