Razgonova Mayya P, Nawaz Muhammad A, Rusakova Elena A, Golokhvast Kirill S
N.I. Vavilov All-Russian Institute of Plant Genetic Resources, B. Morskaya 42-44, 190000 Saint-Petersburg, Russia.
Far Eastern Federal University, Sukhanova 8, 690950 Vladivostok, Russia.
Plants (Basel). 2024 Dec 27;14(1):59. doi: 10.3390/plants14010059.
A comparative metabolomic study of three varieties of wild Rosa (, , and ) from a Kamchatka expedition (2024) was conducted via extraction with supercritical carbon dioxide modified with ethanol (EtOH), and detection of bioactive compounds was realized via tandem mass spectrometry. Several experimental conditions were investigated in the pressure range 50-350 bar, with the used volume of co-solvent ethanol in the amount of 2% in the liquid phase at a temperature in the range of 31-70 °C. The most effective extraction conditions are the following: pressure 200 Bar and temperature 55 °C for ; pressure 250 Bar and temperature 60 °C for ; pressure 200 Bar and temperature 60 °C for . Three varieties of wild contain various phenolic compounds and compounds of other chemical groups with valuable biological activity. Tandem mass spectrometry (HPLC-ESI-ion trap) was applied to detect the target analytes. A total of 283 bioactive compounds (two hundred seventeen compounds from the polyphenol group and sixty-six compounds from other chemical groups) were tentatively identified in extracts from berries of wild . For the first time, forty-eight chemical constituents from the polyphenol group (15 flavones, 14 flavonols, 4 flavan-3-ols, 3 flavanones, 1 phenylpropanoid, 2 gallotannins, 1 ellagitannin, 4 phenolic acids, 1 dihydrochalcone, and 3 coumarins) were identified in supercritical extracts of , , and .
对来自堪察加半岛考察（2024 年）的三种野生蔷薇品种（、和）进行了比较代谢组学研究，采用乙醇改性的超临界二氧化碳萃取法，并通过串联质谱法实现生物活性化合物的检测。在 50 - 350 巴的压力范围内研究了几种实验条件，共溶剂乙醇在液相中的用量为 2%，温度范围为 31 - 70℃。最有效的萃取条件如下：品种的压力为 200 巴，温度为 55℃；品种的压力为 250 巴，温度为 60℃；品种的压力为 200 巴，温度为 60℃。三种野生蔷薇品种含有各种酚类化合物和其他具有重要生物活性的化学基团的化合物。采用串联质谱法（HPLC - ESI - 离子阱）检测目标分析物。在野生蔷薇浆果提取物中初步鉴定出总共 283 种生物活性化合物（多酚类组中有 217 种化合物，其他化学基团中有 66 种化合物）。首次在、和的超临界提取物中鉴定出 48 种多酚类化学成分（15 种黄酮、14 种黄酮醇、4 种黄烷 - 3 - 醇、3 种黄烷酮、1 种苯丙烷类、2 种没食子单宁、1 种鞣花单宁、4 种酚酸、1 种二氢查耳酮和 3 种香豆素）。
