Jessop J J, West G L, Sobotka T J
U.S. Food and Drug Administration, Division of Toxicology, Washington, DC 20204.
J Neuroimmunol. 1989 Dec;25(2-3):241-9. doi: 10.1016/0165-5728(89)90142-2.
Intermittent inescapable footshock (IIFS) treatment, administered to 3-month-old male rats, resulted in analgesia as well as discrete immunological and endocrine changes. The splenic lymphocyte proliferative response to concanavalin A (ConA) and phytohemagglutinin (PHA) was decreased by 20% and 41%, respectively. The primary IgM plaque forming cell (PFC) response to sheep red blood cells (SRBC), however, was not altered by IIFS administered either immediately or 24 h after injection of SRBC. IIFS also produced a significant (20-fold) increase in plasma corticosterone (CORT) as compared to non-shocked controls. The shock-induced suppression of splenic lymphocyte mitogenic response to PHA was blocked by 10 mg/kg naltrexone (NTX) administered immediately before IIFS. NTX alone had no effect on this mitogen response. However, NTX significantly attenuated the shock-induced rise in CORT even though NTX alone significantly elevated CORT in the non-shocked controls. These data suggest that IIFS alters cellular immune response but not the primary IgM PFC response (a measure of humoral immune function) and that the immunomodulatory effects may involve both an opioid and a corticosteroid component with respect to alterations in the splenic lymphocyte mitogenic response to PHA.
对3月龄雄性大鼠施加间歇性不可逃避足部电击（IIFS）治疗，会导致镇痛以及明显的免疫和内分泌变化。脾脏淋巴细胞对刀豆蛋白A（ConA）和植物血凝素（PHA）的增殖反应分别降低了20%和41%。然而，对绵羊红细胞（SRBC）的原发性IgM斑块形成细胞（PFC）反应，在注射SRBC后立即或24小时给予IIFS时均未改变。与未受电击的对照组相比，IIFS还使血浆皮质酮（CORT）显著增加（20倍）。在IIFS之前立即给予10mg/kg纳曲酮（NTX）可阻断电击诱导的脾脏淋巴细胞对PHA的促有丝分裂反应的抑制。单独使用NTX对这种促有丝分裂反应没有影响。然而，NTX显著减弱了电击诱导的CORT升高，尽管单独使用NTX在未受电击的对照组中显著升高了CORT。这些数据表明，IIFS改变细胞免疫反应，但不改变原发性IgM PFC反应（体液免疫功能的一种衡量指标），并且就脾脏淋巴细胞对PHA的促有丝分裂反应的改变而言，免疫调节作用可能涉及阿片类和皮质类固醇成分。
