Kusnecov A W, Rabin B S
Brain, Behavior and Immunity Center, University of Pittsburgh School of Medicine.
J Neuroimmunol. 1993 Apr;44(1):33-42. doi: 10.1016/0165-5728(93)90265-z.
A variety of stressors have been shown to influence specific and non-specific measures of immune function in laboratory animals. One of the most common tools used to evaluate lymphocyte function is the non-specific mitogen proliferation assay. Assessment of this function in the rat spleen has revealed profound suppression following restraint, electric shock, and re-exposure of animals to a fearful context. However, there have been no studies that have compared the effects of stressor exposure on mitogen- and specific antigen-stimulated spleen cell proliferation. Therefore, the present study addressed this issue through experiments in which rats were immunized intraperitoneally with 1 microgram cholera toxin and exposed to acute (one session) or repeated (three consecutive daily sessions) footshock. The results showed that footshock exposure prior to immunization inhibited cholera toxin stimulated spleen cell proliferation 7 days after immunization. Acute or repeated footshock exposure 5-7 days after cholera toxin immunization depressed non-specific spleen cell proliferation, while augmenting the proliferative response to specific antigen. From these observations it can be hypothesized that footshock exposure either differentially regulates lymphocyte activation by clonal and polyclonal signals, and/or naive and memory cells react differently to stressor exposure.
已表明多种应激源会影响实验动物免疫功能的特异性和非特异性指标。用于评估淋巴细胞功能的最常用工具之一是非特异性丝裂原增殖试验。对大鼠脾脏中该功能的评估显示，在限制活动、电击以及使动物再次暴露于恐惧环境后，会出现明显抑制。然而，尚无研究比较应激源暴露对丝裂原和特异性抗原刺激的脾细胞增殖的影响。因此，本研究通过实验解决了这一问题，实验中给大鼠腹腔注射1微克霍乱毒素进行免疫，并使其暴露于急性（一次）或重复（连续三天每日一次）电击。结果显示，免疫前电击暴露会抑制免疫后7天霍乱毒素刺激的脾细胞增殖。霍乱毒素免疫后5 - 7天进行急性或重复电击暴露会抑制非特异性脾细胞增殖，同时增强对特异性抗原的增殖反应。从这些观察结果可以推测，电击暴露要么通过克隆和多克隆信号差异调节淋巴细胞活化，和/或幼稚细胞和记忆细胞对应激源暴露的反应不同。
