Zhao Yun, Zhang Lei, Zhao Hong
Clinical College of Ophthalmology of Tianjin Medical University, Tianjin Eye Hospital and Institute, Tianjin Key Laboratory of Ophthalmology and Vision Science, Tianjin 300020, China.
Clinical College of Ophthalmology of Tianjin Medical University, Tianjin Eye Hospital and Institute, Tianjin Key Laboratory of Ophthalmology and Vision Science, Tianjin 300020, China; Email:
Zhonghua Yan Ke Za Zhi. 2015 Nov;51(11):869-74.
Three-dimensional cell culture technology is a kind of technology that cultures the cells in a three-dimensional cultivation by using a kind of scaffold materials in vitro. Its advantage is that the vivo microenvironment simulating degrees of the three-dimensional culture technology is higher than that of the two-dimensional planar cell culture model, and the controllability is also higher than the animal experiment. In recent years, with the development of tissue engineering technology, a varietiy of the stent of biological materials also a fast development, which provided a favorable platform for three-dimensional cell culture technology. In ophthalmology, three-dimensional cell culture has been applied to the cornea, retina and the visual system development and other optic tumor researches. The objective of this paper is to making a review the application status of the three-dimensional cell culture technology in the basic research and clinical treatment in ophthalmology.
三维细胞培养技术是一种利用支架材料在体外对细胞进行三维培养的技术。其优点是三维培养技术模拟体内微环境的程度高于二维平面细胞培养模型,且可控性也高于动物实验。近年来,随着组织工程技术的发展,各种生物材料支架也得到了快速发展,这为三维细胞培养技术提供了良好的平台。在眼科领域,三维细胞培养已应用于角膜、视网膜以及视觉系统发育和其他眼部肿瘤研究。本文旨在综述三维细胞培养技术在眼科基础研究和临床治疗中的应用现状。