Mazumder Tarikul Huda, Uddin Arif, Chakraborty Supriyo
Department of Biotechnology, Assam University, Silchar 788011, Assam, India.
Department of Biotechnology, Assam University, Silchar 788011, Assam, India.
Genomics. 2016 Apr;107(4):155-61. doi: 10.1016/j.ygeno.2016.02.001. Epub 2016 Feb 2.
GATA2 gene encodes a member of the GATA family of zinc-finger transcription factors that play a pivotal role during the transition of primitive blood forming cells into white blood cells. Mutation in GATA2 results in the loss of function or even gain of function, including abnormal proliferation of white blood cells that may predispose to acute myeloid leukemia. Our results showed that the codon usage in GATA2 has been influenced by GC mutation bias where nature has highly favored fourteen most over represented codons but disfavored the ATA codon across five mammals. Purifying natural selection has affected GATA2 gene in human and other mammals to maintain its protein function during the period of evolution. Our findings report an insight into the codon usage patterns in gaining the clues for codon optimization to alter the translational efficiency as well as for the functional conservation of gene expression and the significance of nucleotide composition in GATA2 gene within mammals.
GATA2基因编码锌指转录因子GATA家族的一个成员，该家族在原始造血细胞向白细胞的转变过程中起关键作用。GATA2基因突变会导致功能丧失甚至功能获得，包括白细胞异常增殖，这可能易患急性髓系白血病。我们的结果表明，GATA2中的密码子使用受到GC突变偏向的影响，在五种哺乳动物中，自然高度偏爱14个最常出现的密码子，但不喜欢ATA密码子。纯化的自然选择在进化过程中影响了人类和其他哺乳动物的GATA2基因，以维持其蛋白质功能。我们的研究结果报告了对密码子使用模式的洞察，为密码子优化以改变翻译效率以及基因表达的功能保守性提供了线索，同时也揭示了哺乳动物GATA2基因中核苷酸组成的重要性。
