Department of Zoology, Moinul Hoque Choudhury Memorial Science College, Algapur, Hailakandi, Assam, 788150, India.
Department of Biotechnology, Assam University, Silchar, Assam, 788011, India.
Mol Neurobiol. 2019 Mar;56(3):1737-1748. doi: 10.1007/s12035-018-1173-y. Epub 2018 Jun 19.
Codon usage bias (CUB) is the non-uniform usage of synonymous codons in which some codons are more preferred to others in the transcript. Analysis of codon usage bias has applications in understanding the basics of molecular biology, genetics, gene expression, and molecular evolution. To understand the patterns of codon usage in genes involved in the central nervous system (CNS), we used bioinformatic approaches to analyze the protein-coding sequences of genes involved in the CNS. The improved effective number of codons (ENC) suggested that the overall codon usage bias was low. The relative synonymous codon usage (RSCU) revealed that the most frequently occurring codons had a G or C at the third codon position. The codons namely TCC, AGC, CTG, CAG, CGC, ATC, ACC, GTG, GCC, GGC, and CGG (average RSCU > 1.6) were over-represented. Both mutation pressure and natural selection might affect the codon usage pattern as evident from correspondence and parity plot analyses. The overall GC content (59.93) was higher than AT content, i.e., genes were GC-rich. The correlation of GC12 with GC3 suggested that mutation pressure might affect the codon usage pattern.
密码子使用偏性（CUB）是指同义密码子使用的非均匀性，其中一些密码子在转录中比其他密码子更受欢迎。密码子使用偏性的分析在理解分子生物学、遗传学、基因表达和分子进化的基本原理方面有应用。为了了解参与中枢神经系统（CNS）的基因中的密码子使用模式，我们使用生物信息学方法来分析参与 CNS 的基因的蛋白质编码序列。改进的有效密码子数（ENC）表明整体密码子使用偏性较低。相对同义密码子使用（RSCU）表明，最常出现的密码子在第三个密码子位置具有 G 或 C。密码子 TCC、AGC、CTG、CAG、CGC、ATC、ACC、GTG、GCC、GGC 和 CGG（平均 RSCU>1.6）过度表达。对应和奇偶校验图分析表明，突变压力和自然选择可能会影响密码子使用模式。总的 GC 含量（59.93）高于 AT 含量，即基因富含 GC。GC12 与 GC3 的相关性表明，突变压力可能会影响密码子使用模式。
