Ngernsoungnern Apichart, Ngernsoungnern Piyada
School of Anatomy, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
School of Anatomy, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Acta Histochem. 2016 Apr;118(3):244-51. doi: 10.1016/j.acthis.2016.01.005. Epub 2016 Feb 2.
Ghrelin is an endogenous hormone detected in the gastrointestinal tracts (GI) of various species. In the present study, ghrelin-like peptide (ghrelin-LP) was identified in the GI tract of the golden apple snail, Pomacea canaliculata. Using immunohistochemistry, the result revealed an immunoreactivity (-ir) of ghrelin-LP in regions of the GI tract. The ghrelin-LP-ir was observed in both opened-type and closed-type cells of the esophagus, stomach, and small and large intestines. The highest density of ghrelin-LP immunoreactive cells was found in the esophagus and the least density was detected in the stomach. The highest percentages of the opened-type and closed-type cells were present in the esophagus and small intestine, respectively. In immunoblotting, the molecular weight of ghrelin-LP was related to the human ghrelin peptide (∼13kDa). Moreover, the concentration of ghrelin-LP was significantly higher in snails that were fasted for 24h compared with fed snails. The concentration decreased after refeeding. The present study could be useful for understanding the physiological role of ghrelin-LP in mollusk species.
胃饥饿素是在多种物种的胃肠道（GI）中检测到的一种内源性激素。在本研究中，在福寿螺（Pomacea canaliculata）的胃肠道中鉴定出了胃饥饿素样肽（ghrelin-LP）。使用免疫组织化学方法，结果显示胃肠道各区域存在胃饥饿素-LP免疫反应性（-ir）。在食管、胃以及小肠和大肠的开放型和封闭型细胞中均观察到胃饥饿素-LP免疫反应性。胃饥饿素-LP免疫反应性细胞密度最高的部位是食管，密度最低的部位是胃。开放型和封闭型细胞比例最高的部位分别是食管和小肠。在免疫印迹中，胃饥饿素-LP的分子量与人类胃饥饿素肽（约13kDa）相关。此外，与喂食的福寿螺相比，禁食24小时的福寿螺中胃饥饿素-LP的浓度显著更高。重新喂食后浓度降低。本研究可能有助于理解胃饥饿素-LP在软体动物物种中的生理作用。
