IHEM, CONICET , Universidad Nacional de Cuyo , Mendoza , Argentina.
Universidad Nacional de Cuyo, Facultad de Ciencias Médicas , Instituto de Fisiología , Mendoza 5500 , Argentina.
J Proteome Res. 2019 Sep 6;18(9):3342-3352. doi: 10.1021/acs.jproteome.9b00282. Epub 2019 Aug 2.
The freshwater snail , an invasive species of global significance, possesses a well-developed digestive system and diverse feeding mechanisms enabling the intake of a wide variety of food. The identification of glycosidases in adult snails would increase the understanding of their digestive physiology and potentially generate new opportunities to eradicate and/or control this invasive species. In this study, liquid chromatography coupled to tandem mass spectrometry was applied to define the occurrence, diversity, and origin of glycoside hydrolases along the digestive tract of . A range of cellulases, hemicellulases, amylases, maltases, fucosidases, and galactosidases were identified across the digestive tract. The digestive gland and the contents of the crop and style sac yield a higher diversity of glycosidase-derived peptides. Subsequently, peptides derived from 81 glycosidases (46 proteins from the public database and 35 uniquely from the transcriptome database) that were distributed among 13 glycoside hydrolase families were selected and quantified using multiple reaction monitoring mass spectrometry. This study showed a high glycosidase abundance and diversity in the gut contents of which participate in extracellular digestion of complex dietary carbohydrates. Salivary and digestive glands were the main tissues involved in their synthesis and secretion.
淡水螺是一种具有全球重要意义的入侵物种，拥有发达的消化系统和多样化的摄食机制，能够摄取各种各样的食物。鉴定成年螺中的糖苷酶将有助于增加对其消化生理学的了解，并可能为根除和/或控制这种入侵物种提供新的机会。在这项研究中，采用液相色谱-串联质谱联用技术来确定沿 的消化道中糖苷水解酶的存在、多样性和来源。在整个消化道中鉴定出了一系列的纤维素酶、半纤维素酶、淀粉酶、麦芽糖酶、岩藻糖苷酶和半乳糖苷酶。消化腺以及前肠和嗉囊内容物产生了更高多样性的糖苷酶衍生肽。随后，使用多重反应监测质谱法选择和定量了 81 种糖苷酶（公共数据库中的 46 种蛋白和转录组数据库中 35 种特有蛋白）衍生的肽，这些糖苷酶分布在 13 种糖苷水解酶家族中。本研究表明， 肠道内容物中存在丰富多样的糖苷酶，这些酶参与复杂膳食碳水化合物的细胞外消化。唾液腺和消化腺是其合成和分泌的主要组织。
