采用玻璃毛细管微流控溶剂置换法制备用于对乙酰氨基酚控释的聚（d，l-丙交酯）/蒙脱石复合纳米颗粒。

Fabrication of composite poly(d,l-lactide)/montmorillonite nanoparticles for controlled delivery of acetaminophen by solvent-displacement method using glass capillary microfluidics.

作者信息

Othman Rahimah, Vladisavljević Goran T, Thomas Noreen L, Nagy Zoltan K

机构信息

Department of Chemical Engineering, Loughborough University, Ashby Road, Loughborough, Leicestershire LE11 3TU, UK; School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, 02600 Arau, Perlis, Malaysia.

Department of Chemical Engineering, Loughborough University, Ashby Road, Loughborough, Leicestershire LE11 3TU, UK.

出版信息

Colloids Surf B Biointerfaces. 2016 May 1;141:187-195. doi: 10.1016/j.colsurfb.2016.01.042. Epub 2016 Jan 27.

DOI:10.1016/j.colsurfb.2016.01.042

PMID:26852102

Abstract

Paracetamol (PCM)-loaded composite nanoparticles (NPs) composed of a biodegradable poly(d,l-lactide) (PLA) polymer matrix filled with organically modified montmorillonite (MMT) nanoparticles were fabricated by antisolvent nanoprecipitation in a microfluidic co-flow glass capillary device. The incorporation of MMT in the polymer improved both the drug encapsulation efficiency and the drug loading, and extended the rate of drug release in simulated intestinal fluid (pH 7.4). The particle size increased on increasing both the drug loading and the concentration of MMT in the polymer matrix, and decreased on increasing the aqueous to organic flow rate ratio. The drug encapsulation efficiency in the NPs was higher at higher aqueous to organic flow rate ratio due to faster formation of the NPs. The PCM-loaded PLA NPs containing 2 wt% MMT in PLA prepared at an aqueous to organic flow rate ratio of 10 with an orifice size of 200 μm exhibited a spherical shape with a mean size of 296 nm, a drug encapsulation efficiency of 38.5% and a drug loading of 5.4%. The encapsulation of MMT and PCM in the NPs was confirmed by transmission electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction, differential scanning calorimetry, thermogravimetric analysis and attenuated total reflection-Fourier transform infrared spectroscopy.

摘要

通过在微流控共流玻璃毛细管装置中采用反溶剂纳米沉淀法，制备了由填充有机改性蒙脱土（MMT）纳米颗粒的可生物降解聚（d，l-丙交酯）（PLA）聚合物基质组成的载对乙酰氨基酚（PCM）复合纳米颗粒（NPs）。在聚合物中加入MMT提高了药物包封效率和载药量，并延长了在模拟肠液（pH 7.4）中的药物释放速率。聚合物基质中载药量和MMT浓度增加时，粒径增大；水相流速与有机相流速之比增加时，粒径减小。由于纳米颗粒形成速度更快，在较高的水相流速与有机相流速之比下，纳米颗粒中的药物包封效率更高。在水相流速与有机相流速之比为10、孔口尺寸为200μm的条件下制备的PLA中含有2 wt% MMT的载PCM的PLA纳米颗粒呈球形，平均尺寸为296 nm，药物包封效率为38.5%，载药量为5.4%。通过透射电子显微镜、能量色散X射线光谱、X射线衍射、差示扫描量热法、热重分析和衰减全反射-傅里叶变换红外光谱证实了MMT和PCM在纳米颗粒中的包封情况。