Aslam Mariam, Barkat Kashif, Malik Nadia Shamshad, Alqahtani Mohammed S, Anjum Irfan, Khalid Ikrima, Tulain Ume Ruqia, Gohar Nitasha, Zafar Hajra, Paiva-Santos Ana Cláudia, Raza Faisal
Faculty of Pharmacy, The University of Lahore, Lahore 54000, Pakistan.
Faculty of Pharmacy, Capital University of Science and Technology (CUST), Islamabad 44000, Pakistan.
Pharmaceutics. 2022 Jun 8;14(6):1218. doi: 10.3390/pharmaceutics14061218.
The objective of this study was to fabricate and evaluate a pH sensitive cross-linked polymeric network through the free radical polymerization technique for the model drug, cyclophosphamide, used in the treatment of non-Hodgkin's lymphoma. The Hydrogels were prepared using a polymeric blend of agarose, Pluronic acid, glutaraldehyde, and methacrylic acid. The prepared hydrogels were characterized for drug loading (%), swelling pattern, release behavior, the ingredient's compatibility, structural evaluation, thermal integrity, and toxicity evaluation in rabbits. The new polymer formation was evident from FTIR findings. The percentage loaded into the hydrogels was in the range of 58.65-75.32%. The developed hydrogels showed significant differences in swelling dynamics and drug release behavior in simulated intestinal fluid (SIF) when compared with simulated gastric fluid (SGF). The drug release was persistent and performed in a controlled manner for up to 24 h. A toxicity study was conducted on white albino rabbits. The developed hydrogels did not show any signs of ocular, skin, or oral toxicity; therefore, these hydrogels can be regarded as safe and potential carriers for controlled drug delivery in biomedical applications.
本研究的目的是通过自由基聚合技术制备并评估一种用于治疗非霍奇金淋巴瘤的模型药物环磷酰胺的pH敏感交联聚合物网络。水凝胶是使用琼脂糖、普朗尼克酸、戊二醛和甲基丙烯酸的聚合物共混物制备的。对制备的水凝胶进行了载药量（%）、溶胀模式、释放行为、成分相容性、结构评估、热完整性以及家兔毒性评估等方面的表征。傅里叶变换红外光谱（FTIR）结果表明形成了新的聚合物。水凝胶中的载药百分比在58.65 - 75.32%范围内。与模拟胃液（SGF）相比，所制备的水凝胶在模拟肠液（SIF）中的溶胀动力学和药物释放行为存在显著差异。药物释放持续进行，并以可控方式持续长达24小时。对白化家兔进行了毒性研究。所制备的水凝胶未表现出任何眼部、皮肤或口服毒性迹象；因此，这些水凝胶可被视为生物医学应用中药物控释的安全且有潜力的载体。
