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通过染色质的动态特性探索其组织机制。

Exploring chromatin organization mechanisms through its dynamic properties.

作者信息

Bronshtein Irena, Kanter Itamar, Kepten Eldad, Lindner Moshe, Berezin Shirly, Shav-Tal Yaron, Garini Yuval

机构信息

a Physics Department and Nanotechnology Institute , Bar Ilan University , Ramat Gan , Israel.

b The Mina & Everard Goodman Faculty of Life Sciences and Nanotechnology Institute, Bar Ilan University , Ramat Gan , Israel.

出版信息

Nucleus. 2016;7(1):27-33. doi: 10.1080/19491034.2016.1139272. Epub 2016 Feb 8.

Abstract

The organization of the genome in the nucleus is believed to be crucial for different cellular functions. It is known that chromosomes fold into distinct territories, but little is known about the mechanisms that maintain these territories. To explore these mechanisms, we used various live-cell imaging methods, including single particle tracking to characterize the diffusion properties of different genomic regions in live cells. Chromatin diffusion is found to be slow and anomalous; in vast contrast, depletion of lamin A protein significantly increases chromatin motion, and the diffusion pattern of chromatin transforms from slow anomalous to fast normal. More than this, depletion of lamin A protein also affects the dynamics of nuclear bodies. Our findings indicate that chromatin motion is mediated by lamin A and we suggest that constrained chromatin mobility allows to maintain chromosome territories. Thus, the discovery of this function of nucleoplasmic lamin A proteins sheds light on the maintenance mechanism of chromosome territories in the interphase nucleus, which ensures the proper function of the genome.

摘要

基因组在细胞核中的组织方式被认为对不同的细胞功能至关重要。已知染色体折叠成不同的区域,但对于维持这些区域的机制却知之甚少。为了探索这些机制,我们使用了各种活细胞成像方法,包括单粒子追踪来表征活细胞中不同基因组区域的扩散特性。发现染色质扩散缓慢且异常;与此形成巨大反差的是,核纤层蛋白A的缺失显著增加了染色质的运动,并且染色质的扩散模式从缓慢异常转变为快速正常。不仅如此,核纤层蛋白A的缺失还会影响核体的动态变化。我们的研究结果表明,染色质运动由核纤层蛋白A介导,并且我们认为受限的染色质流动性有助于维持染色体区域。因此,核质核纤层蛋白A的这一功能的发现揭示了间期细胞核中染色体区域的维持机制,这确保了基因组的正常功能。

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