ARS元件沿酵母三号染色体的复制时间。
Time of replication of ARS elements along yeast chromosome III.
作者信息
Reynolds A E, McCarroll R M, Newlon C S, Fangman W L
机构信息
Department of Genetics, University of Washington, Seattle 98195.
出版信息
Mol Cell Biol. 1989 Oct;9(10):4488-94. doi: 10.1128/mcb.9.10.4488-4494.1989.
Abstract
The replication of putative replication origins (ARS elements) was examined for 200 kilobases of chromosome III of Saccharomyces cerevisiae. By using synchronous cultures and transfers from dense to light isotope medium, the temporal pattern of mitotic DNA replication of eight fragments that contain ARSs was determined. ARS elements near the telomeres replicated late in S phase, while internal ARS elements replicated in the first half of S phase. The results suggest that some ARS elements in the chromosome may be inactive as replication origins. The actively expressed mating type locus, MAT, replicated early in S phase, while the silent cassettes, HML and HMR, replicated late. Unexpectedly, chromosome III sequences were found to replicate late in G1 at the arrest induced by the temperature-sensitive cdc7 allele.
摘要
对酿酒酵母III号染色体200千碱基区域内假定的复制起点（自主复制序列元件）的复制情况进行了研究。通过使用同步培养物以及从高密度同位素培养基转移至低密度同位素培养基，确定了包含自主复制序列元件的八个片段的有丝分裂DNA复制的时间模式。端粒附近的自主复制序列元件在S期后期复制，而内部自主复制序列元件在S期前半段复制。结果表明，染色体中的一些自主复制序列元件可能作为复制起点是无活性的。活跃表达的交配型位点MAT在S期早期复制，而沉默盒HML和HMR则在后期复制。出乎意料的是，在温度敏感型cdc7等位基因诱导的停滞期，III号染色体序列在G1期后期复制。
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