Fangman W L, Hice R H, Chlebowicz-Sledziewska E
Cell. 1983 Mar;32(3):831-8. doi: 10.1016/0092-8674(83)90069-7.
A 1.45 kb circular plasmid derived from yeast chromosome IV contains the autonomous replication element called ARS1. Isotope density transfer experiments show that each plasmid molecule replicates once each S phase, with initiation depending on two genetically defined steps required for nuclear DNA replication. A density transfer experiment with synchronized cells demonstrates that the ARS1 plasmid population replicates early in the S phase. The sequences adjacent to ARS1 on chromosome IV also initiate replication early, suggesting that the ARS1 plasmid contains information which determines its time of replication. The times of replication for two other yeast chromosome sequences, ARS2 and a sequence referred to as 1OZ, indicate that the temporal order of replication is ARS1 leads to ARS2 leads to 1OZ. These experiments show directly that specific chromosome regions replicate at specific times during the yeast S phase. If ARS elements are origins of chromosome replication, then the experiment reveals times of activation for two origins.
一个源自酵母四号染色体的1.45 kb环状质粒含有名为ARS1的自主复制元件。同位素密度转移实验表明，每个质粒分子在每个S期复制一次，起始依赖于核DNA复制所需的两个遗传学定义步骤。对同步化细胞进行的密度转移实验表明，ARS1质粒群体在S期早期进行复制。四号染色体上与ARS1相邻的序列也在S期早期起始复制，这表明ARS1质粒含有决定其复制时间的信息。另外两个酵母染色体序列ARS2和一个称为1OZ的序列的复制时间表明，复制的时间顺序是ARS1 → ARS2 → 1OZ。这些实验直接表明，在酵母S期的特定时间，特定的染色体区域会进行复制。如果ARS元件是染色体复制的起始点，那么该实验揭示了两个起始点的激活时间。
