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Upstream regulatory sequences of the yeast RNR2 gene include a repression sequence and an activation site that binds the RAP1 protein.

作者信息

Hurd H K, Roberts J W

机构信息

Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853.

出版信息

Mol Cell Biol. 1989 Dec;9(12):5359-72. doi: 10.1128/mcb.9.12.5359-5372.1989.

DOI:10.1128/mcb.9.12.5359-5372.1989
PMID:2685560
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363704/
Abstract

The small subunit of ribonucleotide reductase in Saccharomyces cerevisiae (RNR2) was induced 3- to 20-fold by a variety of DNA-damaging agents. Induction of the RNR2 transcript by at least one of these agents, methyl methanesulfonate, did not require protein synthesis. To identify sequences involved in the regulation of RNR2, we introduced deletions upstream of the transcription start site. Sequences required for induction were contained within a 200-base-pair region that could confer methyl methanesulfonate inducibility on the heterologous CYC1 promoter. This region contained a repression sequence and at least two positive activation sites. One of these activation sites bound RAP1, a protein known to associate with mating-type silencers and the upstream activation sequences of a number of genes. The behavior of deletions of the repression sequence suggests that induction of RNR2 may occur, at least in part, through relief of repression.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/363704/25fc69d60266/molcellb00060-0104-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/363704/1ccd7d680100/molcellb00060-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/363704/535116bd7da0/molcellb00060-0099-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/363704/18fcb9c8555c/molcellb00060-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/363704/1df40be00bc2/molcellb00060-0103-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/363704/1d5c0d00e3a1/molcellb00060-0104-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/363704/25fc69d60266/molcellb00060-0104-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/363704/1ccd7d680100/molcellb00060-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/363704/535116bd7da0/molcellb00060-0099-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/363704/18fcb9c8555c/molcellb00060-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/363704/1df40be00bc2/molcellb00060-0103-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/363704/1d5c0d00e3a1/molcellb00060-0104-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/363704/25fc69d60266/molcellb00060-0104-b.jpg

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本文引用的文献

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Deoxyribonucleotide biosynthesis in yeast (Saccharomyces cerevisiae). A ribonucleotide reductase system of sufficient activity for DNA synthesis.酵母（酿酒酵母）中的脱氧核糖核苷酸生物合成。一种具有足够活性用于DNA合成的核糖核苷酸还原酶系统。
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