Ma Jun, Liu Fang, Wang Qinglian, Wang Kunbo, Jones Don C, Zhang Baohong
Department of Biology, East Carolina University, Greenville, NC 27858, USA.
State Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan 455000, P. R. China.
Sci Rep. 2016 Feb 9;6:21535. doi: 10.1038/srep21535.
TCP proteins are plant-specific transcription factors implicated to perform a variety of physiological functions during plant growth and development. In the current study, we performed for the first time the comprehensive analysis of TCP gene family in a diploid cotton species, Gossypium arboreum, including phylogenetic analysis, chromosome location, gene duplication status, gene structure and conserved motif analysis, as well as expression profiles in fiber at different developmental stages. Our results showed that G. arboreum contains 36 TCP genes, distributing across all of the thirteen chromosomes. GaTCPs within the same subclade of the phylogenetic tree shared similar exon/intron organization and motif composition. In addition, both segmental duplication and whole-genome duplication contributed significantly to the expansion of GaTCPs. Many these TCP transcription factor genes are specifically expressed in cotton fiber during different developmental stages, including cotton fiber initiation and early development. This suggests that TCP genes may play important roles in cotton fiber development.
TCP蛋白是植物特有的转录因子，在植物生长发育过程中发挥多种生理功能。在本研究中，我们首次对二倍体棉花物种亚洲棉的TCP基因家族进行了全面分析，包括系统发育分析、染色体定位、基因复制状态、基因结构和保守基序分析，以及不同发育阶段纤维中的表达谱。我们的结果表明，亚洲棉含有36个TCP基因，分布在全部13条染色体上。系统发育树同一亚分支内的GaTCPs具有相似的外显子/内含子组织和基序组成。此外，片段重复和全基因组重复对GaTCPs的扩增有显著贡献。许多这些TCP转录因子基因在棉花纤维不同发育阶段特异性表达，包括棉花纤维起始和早期发育阶段。这表明TCP基因可能在棉花纤维发育中发挥重要作用。
