Hao Juan, Lou Panpan, Han Yidie, Chen Zhehao, Chen Jianmei, Ni Jun, Yang Yanjun, Jiang Zhifang, Xu Maojun
Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou, China.
Front Plant Sci. 2021 Nov 22;12:769675. doi: 10.3389/fpls.2021.769675. eCollection 2021.
TCP transcription factors play important roles in diverse aspects of plant development as transcriptional activators or repressors. However, the functional mechanisms of TCPs are not well understood, especially in cotton fibers. Here, we identified a total of 37 non-redundant TCP proteins from the diploid cotton (), which showed great diversity in the expression profile. GrTCP11, an ortholog of AtTCP11, was preferentially expressed in cotton anthers and during fiber initiation and secondary cell wall synthesis stages. Overexpression of in reduced root hair length and delayed flowering. It was found that negatively regulated genes involved in jasmonic acid (JA) biosynthesis and response, such as , , , , , , , and , which resulted in a decrease in JA concentration in the overexpressed transgenic lines. As with the JA-deficient mutant , the transgenic line 4-1 was insensitive to 50 μM methyl jasmonate, compared with the wild-type plants. The results suggest that GrTCP11 may be an important transcription factor for cotton fiber development, by negatively regulating JA biosynthesis and response.
TCP转录因子作为转录激活因子或抑制因子，在植物发育的多个方面发挥着重要作用。然而，TCPs的功能机制尚未完全清楚，尤其是在棉花纤维中。在此，我们从二倍体棉花中总共鉴定出37个非冗余的TCP蛋白，它们在表达谱上表现出很大的差异。GrTCP11是拟南芥AtTCP11的直系同源基因，在棉花花药中以及纤维起始和次生细胞壁合成阶段优先表达。在拟南芥中过表达GrTCP11会缩短根毛长度并延迟开花。研究发现，GrTCP11负调控参与茉莉酸（JA）生物合成和响应的基因，如LOX2、AOS、AOC、OPR3、JAR1、MYC2、PDF1.2和VSP2，这导致过表达转基因株系中JA浓度降低。与JA缺陷型突变体一样，与野生型植株相比，转基因株系4-1对50μM茉莉酸甲酯不敏感。结果表明，GrTCP11可能是棉花纤维发育的重要转录因子，通过负调控JA生物合成和响应来实现。
