Chen Jie, Hu Lijuan, Chen Jian, Pan Qinshi, Ding Hongyan, Xu Gang, Zhu Peiwu, Wen Xiusu, Huang Keta, Wang Yumin
Department of Intensive Care Unit, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang Province, People's Republic of China.
Pathol Oncol Res. 2016 Jul;22(3):609-15. doi: 10.1007/s12253-016-0046-9. Epub 2016 Feb 9.
Studies have shown that the expression profile of Wnt signaling pathway is very important in lung adenocarcinoma (LAD) and some lncRNAs can regulate the expression of key molecules of Wnt pathway. However, Wnt pathway related lncRNAs are not systematically analyzed and detected in lung adenocarcinoma. We used a high-throughput microarray to compare the lncRNA expression profiles in LAD and corresponding normal tissue (NT) samples. Several candidate Wnt pathway related lncRNAs were verified by real-time quantitative reverse transcription polymerase chain reaction (PCR) analysis. We found that 232 Wnt pathway related lncRNAs were obviously expressed (≥2-fold change) in lung adenocarcinoma samples and 13 Wnt pathway related lncRNAs were aberrantly expressed in lung adenocarcinoma compared with matched histologically normal lung tissues by qPCR. Among these, RP11-181G12.2 and RP11-89 K21.1 were the most aberrantly expressed lncRNAs. Our study ascertained the expression of Wnt pathway related lncRNAs in lung adenocarcinoma. The results revealed that many Wnt pathway related lncRNAs were differentially expressed in lung adenocarcinoma tissues, suggesting that they may play a key role in tumor development.
研究表明，Wnt信号通路的表达谱在肺腺癌（LAD）中非常重要，一些长链非编码RNA（lncRNA）可以调节Wnt通路关键分子的表达。然而，在肺腺癌中尚未对Wnt通路相关lncRNA进行系统的分析和检测。我们使用高通量微阵列比较了肺腺癌和相应正常组织（NT）样本中的lncRNA表达谱。通过实时定量逆转录聚合酶链反应（PCR）分析验证了几种候选的Wnt通路相关lncRNA。我们发现，与配对的组织学正常肺组织相比，232种Wnt通路相关lncRNA在肺腺癌样本中明显表达（≥2倍变化），13种Wnt通路相关lncRNA在肺腺癌中异常表达。其中，RP11-181G12.2和RP11-89 K21.1是表达异常最明显的lncRNA。我们的研究确定了Wnt通路相关lncRNA在肺腺癌中的表达情况。结果显示，许多Wnt通路相关lncRNA在肺腺癌组织中差异表达，提示它们可能在肿瘤发生发展中起关键作用。
