Wei Furong, Wang Qianrong, Su Qinghong, Huang Haiyan, Luan Junwen, Xu Xiaoqun, Wang Junfu
Institute of Basic Medicine, Shandong Academy of Medical Sciences, School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, 18877 Jingshi Road, 250062, Jinan, Shandong, China.
Department of Radiation Oncology, Shandong Cancer Hospital, Shandong Academy of Medical Sciences, 440 Jiyan Road, Jinan, China.
Cell Mol Neurobiol. 2016 Nov;36(8):1389-1397. doi: 10.1007/s10571-016-0338-3. Epub 2016 Feb 8.
Glioblastoma multiforme (GBM) is the most malignant glioma, unveiling the underlying mechanisms of its aggressiveness could promote the discovery of potential targets for effective treatment. MicroRNAs (miRNAs) are important participants in both development and disease, its involvement in cancers has long been recognized. In this study, we investigated the role of miRNA-373 (miR-373) in GBM cell line U251, demonstrated that although miR-373 does not affect cell growth of U251, it inhibits migration and invasion of U251. Forced expression of miR-373 down-regulates the expressions CD44 and TGFBR2, while knockdown of CD44 and TGFBR2 presents the similar phenotype as miR-373 overexpression, suggesting that CD44 and TGFBR2 are functional targets of miR-373, down-regulation of CD44 and TGFBR2 by miR-373 are partly responsible for the migration, and invasion suppressive role of miR-373 in U251.
多形性胶质母细胞瘤（GBM）是最恶性的胶质瘤，揭示其侵袭性的潜在机制可促进有效治疗潜在靶点的发现。微小RNA（miRNA）是发育和疾病中的重要参与者，其在癌症中的作用早已得到认可。在本研究中，我们研究了miRNA-373（miR-373）在GBM细胞系U251中的作用，证明尽管miR-373不影响U251的细胞生长，但它抑制U251的迁移和侵袭。miR-373的强制表达下调CD44和TGFBR2的表达，而敲低CD44和TGFBR2呈现与miR-373过表达相似的表型，表明CD44和TGFBR2是miR-373的功能靶点，miR-373对CD44和TGFBR2的下调部分负责miR-373在U251中的迁移和侵袭抑制作用。
