Katayama Akihiro, Noguchi Hirofumi, Kuise Takashi, Nakatsuka Atsuko, Hirota Daisho, Kataoka Hitomi Usui, Kawai Takashi, Inoue Kentaro, Imagawa Noriko, Saitoh Issei, Noguchi Yasufumi, Watanabe Masami, Wada Jun, Fujiwara Toshiyoshi
Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama 700-8558 , Japan.
† Department of Surgery, Chiba-East National Hospital, National Hospital Organization , Chiba 260-8712 , Japan.
Cell Med. 2013 Oct 21;6(1-2):3-8. doi: 10.3727/215517913X674171. eCollection 2013 Dec 30.
For pancreatic islet transplantation, maintaining organ viability after pancreas procurement is critical and a major determinant for better graft function and survival. University of Wisconsin (UW) solution is currently the gold standard for abdominal organ preservation and the pancreas in particular. However, in the use of UW preservation solution for islet transplantation, there are disadvantages to be overcome, such as the inhibition of collagenase activity during pancreatic digestion. In this study, we compared UW solution with HN-1 solution in pancreas preservation for islet isolation. Islet yield was significantly greater in the HN-1 group than the UW group both before and after purification. In the in vitro assay, the adenosine triphosphate content in cultured islets was significantly higher in the HN-1 group than in the UW group. Furthermore, in streptozotocin-induced diabetic nude mice, the islet graft function of the HN-1 group was superior to that of the UW group. We concluded that the use of HN-1 solution is a promising approach for optimal pancreas preservation in islet transplantation.
对于胰岛移植而言，在获取胰腺后维持器官活力至关重要，且是移植器官功能更佳及存活时间更长的一个主要决定因素。威斯康星大学（UW）溶液目前是腹部器官保存（尤其是胰腺保存）的金标准。然而，在将UW保存液用于胰岛移植时，仍有一些缺点有待克服，比如在胰腺消化过程中会抑制胶原酶活性。在本研究中，我们比较了UW溶液和HN-1溶液在胰岛分离胰腺保存中的效果。在纯化前后，HN-1组的胰岛产量均显著高于UW组。在体外试验中，HN-1组培养胰岛中的三磷酸腺苷含量显著高于UW组。此外，在链脲佐菌素诱导的糖尿病裸鼠中，HN-1组的胰岛移植功能优于UW组。我们得出结论，使用HN-1溶液是胰岛移植中实现胰腺最佳保存的一种有前景的方法。
