Nahar Saifun, Nakashima Yoshiki, Miyagi-Shiohira Chika, Kinjo Takao, Kobayashi Naoya, Saitoh Issei, Watanabe Masami, Noguchi Hirofumi, Fujita Jiro
Department of Infectious, Respiratory, and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa 903-0215, Japan.
Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa 903-0215, Japan.
Stem Cells Int. 2018 Sep 6;2018:1625464. doi: 10.1155/2018/1625464. eCollection 2018.
Preservation of adipose tissue before the isolation of cells is one of the most important steps in maintaining the cell viability of adipose tissue-derived mesenchymal stem cells (ADSCs) for clinical use. Hank's balanced salt solution (HBSS) is one of the main ADSC preservation solutions used clinically. However, this step is known to lead to decreased cell viability. The University of Wisconsin (UW) solution is recognized by transplant physicians as an excellent organ preservation solution. We aimed to investigate the effectiveness of UW solution in preservation of the viability of ADSCs. We collected adipose tissue from the inguinal fat pad of mice and compared preservation in UW solution and HBSS overnight by measuring cell viability after isolation. We found that the number of viable cells harvested per gram of adipose tissue mass was higher in UW solution- than HBSS-preserved tissue.
在分离细胞之前保存脂肪组织是维持用于临床的脂肪组织来源间充质干细胞（ADSCs）细胞活力的最重要步骤之一。汉克平衡盐溶液（HBSS）是临床上使用的主要ADSC保存溶液之一。然而，已知这一步骤会导致细胞活力下降。威斯康星大学（UW）溶液被移植医生认为是一种出色的器官保存溶液。我们旨在研究UW溶液在保存ADSCs活力方面的有效性。我们从小鼠腹股沟脂肪垫收集脂肪组织，并通过在分离后测量细胞活力来比较在UW溶液和HBSS中过夜保存的情况。我们发现，每克脂肪组织质量收获的活细胞数量在UW溶液保存的组织中比HBSS保存的组织中更高。
