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基质辅助激光解吸电离飞行时间质谱技术对从 chromID C. difficile 显色琼脂中分离出的艰难梭菌进行正确鉴定的重要性。

The importance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for correct identification of Clostridium difficile isolated from chromID C. difficile chromogenic agar.

机构信息

Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China.

Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China; Infection Control Team, Queen Mary Hospital, Hong Kong Special Administrative Region, China.

出版信息

J Microbiol Immunol Infect. 2017 Oct;50(5):723-726. doi: 10.1016/j.jmii.2015.12.002. Epub 2016 Jan 11.

DOI:10.1016/j.jmii.2015.12.002
PMID:26860352
Abstract

The clinical workflow of using chromogenic agar and matrix-assisted laser desorption ionization time-of-fight mass spectrometry (MALDI-TOF MS) for Clostridium difficile identification was evaluated. The addition of MALDI-TOF MS identification after the chromID C. difficile chromogenic agar culture could significantly improve the diagnostic accuracy of C. difficile.

摘要

评估了使用显色琼脂和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）进行艰难梭菌鉴定的临床工作流程。在 chromID C. difficile 显色琼脂培养后添加 MALDI-TOF MS 鉴定，可以显著提高艰难梭菌的诊断准确性。

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