Kamou Nathalie N, Dubey Mukesh, Tzelepis Georgios, Menexes Georgios, Papadakis Emmanouil N, Karlsson Magnus, Lagopodi Anastasia L, Jensen Dan Funck
Laboratory of Plant Pathology, School of Agriculture, Aristotle University of Thessaloniki, P.O. Box 269, 541 24, Thessaloniki, Greece.
Department of Forest Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Box 7026, 75007, Uppsala, Sweden.
Arch Microbiol. 2016 May;198(4):369-77. doi: 10.1007/s00203-016-1198-4. Epub 2016 Feb 9.
This study was carried out to assess the compatibility of the biocontrol fungus Clonostachys rosea IK726 with the phenazine-producing Pseudomonas chlororaphis ToZa7 or with the prodigiosin-producing Serratia rubidaea S55 against Fusarium oxysporum f. sp. radicis-lycopersici. The pathogen was inhibited by both strains in vitro, whereas C. rosea displayed high tolerance to S. rubidaea but not to P. chlororaphis. We hypothesized that this could be attributed to the ATP-binding cassette (ABC) proteins. The results of the reverse transcription quantitative PCR showed an induction of seven genes (abcB1, abcB20, abcB26, abcC12, abcC12, abcG8 and abcG25) from subfamilies B, C and G. In planta experiments showed a significant reduction in foot and root rot on tomato plants inoculated with C. rosea and P. chlororaphis. This study demonstrates the potential for combining different biocontrol agents and suggests an involvement of ABC transporters in secondary metabolite tolerance in C. rosea.
本研究旨在评估生防真菌粉红粘帚霉IK726与产吩嗪的绿针假单胞菌ToZa7或产灵菌红素的类产碱假单胞菌S55对番茄根腐尖孢镰刀菌的兼容性。两种菌株在体外均能抑制该病原菌，而粉红粘帚霉对类产碱假单胞菌具有高耐受性，但对绿针假单胞菌不具有高耐受性。我们推测这可能归因于ATP结合盒（ABC）蛋白。逆转录定量PCR结果显示，来自B、C和G亚家族的7个基因（abcB1、abcB20、abcB26、abcC12、abcC12、abcG8和abcG25）被诱导。在植物体内实验中，接种粉红粘帚霉和绿针假单胞菌的番茄植株的根腐病显著减轻。本研究证明了组合不同生防剂的潜力，并表明ABC转运蛋白参与了粉红粘帚霉对次生代谢产物的耐受性。
