Majewska Anna, Lasek Witold, Janyst Michał, Młynarczyk Grażyna
Chair and Department of Medical Microbiology, Medical University of Warsaw, Poland.
Department of Immunology, Center of Biostructure Research, Medical University of Warsaw, Poland.
Cent Eur J Immunol. 2015;40(4):395-9. doi: 10.5114/ceji.2015.56960. Epub 2016 Jan 15.
There are no specific antivirals designed for adenoviral infections. Due to many cases of adenovirus infections worldwide, epidemic nature of some types of adenoviruses, and growing number of patients with severe adenoviral infections resulting from dysfunction the immune system, the need for searching an effective and safe therapy is increasing. Inosine pranobex exerts antiviral effects which are both direct and secondary to immunomodulatory activity. In the present study we evaluated in vitro effect of inosine pranobex and interferon α (IFN-α) on replication of HAdV-2 and HAdV-5. The effectiveness of inosine pranobex under these conditions has not been previously reported. In conducted study we reported that inosine pranobex reduced the titer of infectious HAdV-2 and HAdV-5 in vitro. Higher concentrations of IP strongly inhibited multiplication of viruses. Combination of inosine pranobex and IFN-α display higher efficacy than either treatment alone and suggest that both agents may increase therapeutic effectiveness without augmenting toxic effects. Combination index calculations showed that inosine pranobex and INF-α synergistically inhibit HAdV-2 and HAdV-5 titers in A549 cells.
目前尚无专门针对腺病毒感染设计的特效抗病毒药物。鉴于全球范围内腺病毒感染病例众多、部分腺病毒具有流行性，以及因免疫系统功能障碍导致的严重腺病毒感染患者数量不断增加，寻找有效且安全的治疗方法的需求日益迫切。异丙肌苷具有直接抗病毒作用以及继发于免疫调节活性的抗病毒作用。在本研究中，我们评估了异丙肌苷和干扰素α（IFN-α）对人腺病毒2型（HAdV-2）和人腺病毒5型（HAdV-5）复制的体外作用。此前尚未有关于在此条件下异丙肌苷有效性的报道。在本研究中我们发现，异丙肌苷在体外可降低感染性HAdV-2和HAdV-5的滴度。较高浓度的异丙肌苷能强烈抑制病毒增殖。异丙肌苷与IFN-α联合使用的疗效高于单独使用任一药物，这表明两种药物联合使用可能在不增加毒性作用的情况下提高治疗效果。联合指数计算表明，异丙肌苷和INF-α在A549细胞中协同抑制HAdV-2和HAdV-5的滴度。
