苏丹喀土穆州先天性异常婴儿中人类巨细胞病毒（HCMV）的分子检测

Molecular Detection of Human Cytomegalovirus (HCMV) Among Infants with Congenital Anomalies in Khartoum State, Sudan.

作者信息

Ebrahim Maha G, Ali Aisha S, Mustafa Mohamed O, Musa Dalal F, El Hussein Abdel Rahim M, Elkhidir Isam M, Enan Khalid A

机构信息

Department of Microbiology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan.

Department of Microbiology, Faculty of Medical Laboratory Sciences, National University, Sudan.

出版信息

Open Virol J. 2015 Dec 10;9:38-41. doi: 10.2174/1874357901509010038. eCollection 2015.

DOI:10.2174/1874357901509010038

PMID:26862356

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4740961/

Abstract

Human Cytomegalovirus (HCMV) infection still represents the most common potentially serious viral complication in humans and is a major cause of congenital anomalies in infants. This study is aimed to detect HCMV in infants with congenital anomalies. Study subjects consisted of infants born with neural tube defect, hydrocephalus and microcephaly. Fifty serum specimens (20 males, 30 females) were collected from different hospitals in Khartoum State. The sera were investigated for cytomegalovirus specific immunoglobin M (IgM) antibodies using enzyme-linked immunosorbent assay (ELISA), and for Cytomegalovirus DNA using polymerase chain reaction (PCR). Out of the 50 sera tested, one patient's (2%) sample showed HCMV IgM, but with no detectable DNA, other 4(8.2 %) sera were positive for HCMV DNA but with no detectable IgM. Various diagnostic techniques should be considered to evaluate HCMV disease and routine screening for HCMV should be introduced for pregnant women in this setting. It is vital to initiate further research work with many samples from different area to assess prevalence and characterize HCMV and evaluate its maternal health implications.

摘要

人巨细胞病毒（HCMV）感染仍是人类最常见的潜在严重病毒并发症，也是婴儿先天性异常的主要原因。本研究旨在检测先天性异常婴儿中的HCMV。研究对象包括患有神经管缺陷、脑积水和小头畸形的婴儿。从喀土穆州不同医院收集了50份血清标本（20例男性，30例女性）。使用酶联免疫吸附测定（ELISA）检测血清中的巨细胞病毒特异性免疫球蛋白M（IgM）抗体，并使用聚合酶链反应（PCR）检测巨细胞病毒DNA。在检测的50份血清中，1例患者（2%）的样本显示HCMV IgM阳性，但未检测到DNA，其他4份（8.2%）血清HCMV DNA阳性，但未检测到IgM。应考虑采用各种诊断技术来评估HCMV疾病，并应在此环境中对孕妇进行HCMV常规筛查。开展进一步研究工作，从不同地区采集大量样本，以评估HCMV的流行情况、特征及其对孕产妇健康的影响至关重要。