Wickner S
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2815-9. doi: 10.1073/pnas.74.7.2815.
Escherichia coli dnaG protein is involved in the initiation of DNA synthesis dependent on G4 or ST-1 single-stranded phage DNAs [Bouche, J.-P., Zechel, K & Kornberg, A. (1975) J. Biol. Chem. 250, 5995-6001]. The reaction occurs by the following mechanism: dnaG protein binds to specific sites on the DNA in a reaction requiring E. coli DNA binding protein. An oligonucleotide is synthesized in a reaction involving dnaG protein, DNA binding protein, and DNA. With G4 DNA this reaction requires ADP, dTTP (or UTP), and dGTP (or GTP). Elongation of the oligonucleotide can be catalyzed by DNA polymerase II or III in combination with dnaZ protein and DNA elongation factors I and III, presumably by the mechanism previously reported [Wickner, S. (1976) Proc. Natl. Acad. Sci. USA 73, 3511-3515] or by DNA polymerase I.
大肠杆菌dnaG蛋白参与依赖G4或ST - 1单链噬菌体DNA的DNA合成起始过程[布歇，J.-P.，泽歇尔，K和科恩伯格，A.（1975年）《生物化学杂志》250，5995 - 6001]。该反应通过以下机制发生：在需要大肠杆菌DNA结合蛋白的反应中，dnaG蛋白与DNA上的特定位点结合。在涉及dnaG蛋白、DNA结合蛋白和DNA的反应中合成寡核苷酸。对于G4 DNA，该反应需要ADP、dTTP（或UTP）和dGTP（或GTP）。寡核苷酸的延伸可由DNA聚合酶II或III与dnaZ蛋白以及DNA延伸因子I和III联合催化，推测是通过先前报道的机制[维克纳，S.（1976年）《美国国家科学院院刊》73，3511 - 3515]，或者由DNA聚合酶I催化。
