用纯化的大肠杆菌蛋白将φX174病毒DNA转化为双链形式。
Conversion of phiX174 viral DNA to double-stranded form by purified Escherichia coli proteins.
作者信息
Wickner S, Hurwitz J
出版信息
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4120-4. doi: 10.1073/pnas.71.10.4120.
Abstract
The E. coli proteins that catalyze the conversion of varphiX174 single-stranded DNA to duplex DNA have now been purified extensively. The reaction depends on dnaB, dnaC(D), dnaE, and dnaG gene products, DNA elongation factors I and II, E. coli DNA binding protein, and two additional E. coli proteins, replication factors X and Y. DNA synthesis by these proteins requires varphiX174 viral DNA, dNTPs, Mg(+2), and ATP. The product synthesized is full-length linear varphiX174 DNA. The reaction has been resolved into two steps. The first step involves the interaction of ATP and varphiX174 DNA with dnaB and dnaC(D) gene products, E. coli DNA binding protein, and replication factors X and Y in the absence of dNTPs. Subsequent dNMP incorporation requires the addition of DNA polymerase III, DNA elongation factors I and II, dnaG gene product, and dNTPs.
摘要
现在已经对催化φX174单链DNA转化为双链DNA的大肠杆菌蛋白质进行了广泛纯化。该反应依赖于dnaB、dnaC(D)、dnaE和dnaG基因产物、DNA延伸因子I和II、大肠杆菌DNA结合蛋白以及另外两种大肠杆菌蛋白质，即复制因子X和Y。这些蛋白质进行DNA合成需要φX174病毒DNA、脱氧核苷三磷酸（dNTPs）、镁离子（Mg(+2)）和三磷酸腺苷（ATP）。合成的产物是全长线性φX174 DNA。该反应已分解为两个步骤。第一步涉及在没有dNTPs的情况下，ATP和φX174 DNA与dnaB和dnaC(D)基因产物、大肠杆菌DNA结合蛋白以及复制因子X和Y相互作用。随后的脱氧单磷酸核苷（dNMP）掺入需要添加DNA聚合酶III、DNA延伸因子I和II、dnaG基因产物以及dNTPs。
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