不同DNA的体外复制分析。

Analysis of in vitro replication of different DNAs.

作者信息

Hurwitz J

出版信息

CRC Crit Rev Biochem. 1979 Nov;7(1):45-74. doi: 10.3109/10409237909102569.

Abstract

The conversion of single-stranded circular DNA to duplex DNA in vitro occurs by at least three different mechanisms. These differences reside in the manner of priming of these DNAs. In contrast, the elongation of primed DNA templates is a general reaction. A number of these proteins have been isolated and further characterized. In addition, cell-free preparations capable of supporting phi X RFI DNA replication as well as the synthesis of progeny viral phi X174 single-stranded circular DNA have been prepared.

摘要

体外单链环状DNA转化为双链DNA至少通过三种不同机制发生。这些差异存在于这些DNA的引发方式中。相比之下，引发的DNA模板的延伸是一个普遍反应。已经分离并进一步表征了许多此类蛋白质。此外，还制备了能够支持φX RFI DNA复制以及子代病毒φX174单链环状DNA合成的无细胞制剂。