Kloppel T M, Keenan T W, Freeman M J, Morré D J
Proc Natl Acad Sci U S A. 1977 Jul;74(7):3011-3. doi: 10.1073/pnas.74.7.3011.
In mice bearing transplantable mammary carcinomas, serum levels of sialic acid-containing glycolipids were elevated 2.5-fold in pooled serum samples from which gangliosides were purified by column chromatography. A method is also described by which ganglioside content was estimated on as little as 1.0 ml of whole blood to permit studies with individual tumor-bearing mice and age- and litter-matched controls. Using this method, we observed similar elevations in ganglioside levels that were independent of age and sex of the animal and appeared in advance of palpable tumors. Following excision of the tumors, the glycolipid sialic acid values dropped below control levels and remained there. Serum sialic acid of the glycolipid fraction was elevated nearly 2-fold in human carcinoma patients and appeared to decline after surgery.
在患有可移植性乳腺癌的小鼠中，通过柱色谱法从合并的血清样本中纯化神经节苷脂后，含唾液酸糖脂的血清水平升高了2.5倍。还描述了一种方法，通过该方法可在仅1.0毫升全血中估计神经节苷脂含量，以便对荷瘤小鼠个体以及年龄和窝别匹配的对照进行研究。使用该方法，我们观察到神经节苷脂水平有类似升高，这与动物的年龄和性别无关，且在可触及肿瘤出现之前就已出现。切除肿瘤后，糖脂唾液酸值降至对照水平以下并维持在该水平。在人类癌症患者中，糖脂部分的血清唾液酸升高了近2倍，并且在手术后似乎有所下降。
