通过测定甲羟戊酸向胆固醇的分数转化率来测量人体每日胆固醇合成率。
Measurement of daily cholesterol synthesis rates in man by assay of the fractional conversion of mevalonic acid to cholesterol.
作者信息
McNamara D J, Ahrens E H, Samuel P, Crouse J R
出版信息
Proc Natl Acad Sci U S A. 1977 Jul;74(7):3043-6. doi: 10.1073/pnas.74.7.3043.
A significant correlation has been found in man between total daily cholesterol synthesis rates as determined by sterol balance measurements and the fraction of intravenously administered R-[5-14C]mevalonic acid converted to cholesterol. In 30 studies it was found that the mean daily cholesterol synthesis rates estimated by sterol balance measurements ranged from 395 to 3047 mg/day, whereas the fractional conversion of mevalonate to cholesterol varied from 0.28 to 0.99. The two parameters correlated with a coefficient of 0.87, P less than 0.001. This method for estimating cholesterol synthesis rates requires low doses of radioisotopic materials (25 muCi of [14C]mevalonate and 5 muCi of [3H]cholesterol) and less than 1 hr of the patient's time; it can be repeated at intervals of 3 weeks and reflects cholesterol synthesis over a short period of time.
通过固醇平衡测量确定的每日总胆固醇合成率与静脉注射的R-[5-¹⁴C]甲羟戊酸转化为胆固醇的比例之间，在人体中已发现存在显著相关性。在30项研究中发现，通过固醇平衡测量估计的每日平均胆固醇合成率在395至3047毫克/天之间，而甲羟戊酸转化为胆固醇的比例则在0.28至0.99之间。这两个参数的相关系数为0.87，P小于0.001。这种估计胆固醇合成率的方法需要低剂量的放射性同位素材料（25微居里的[¹⁴C]甲羟戊酸和5微居里的[³H]胆固醇），且患者只需不到1小时的时间；它可以每3周重复一次，并反映短时间内的胆固醇合成情况。
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