Windt M L, Bouic P J, Lombard C J, Menkveld R, Kruger T F
Department of Obstetrics and Gynaecology, Tygerberg Hospital, Republic of South Africa.
Arch Androl. 1989;23(2):139-45. doi: 10.3109/01485018908986836.
Enzyme-linked immunosorbent assays (ELISAs) to detect sperm antibodies can be a useful addition to other tests. ELISAs allow more quantitative and detailed information than some other tests, but results should be compared to those of other tests. This study was conducted to correlate results of an ELISA that used a sperm membrane extract as antigen with those of other tests. Semen, seminal plasma, and serum of 34 sperm-antibody-positive and 36 sperm-antibody-negative men were tested. There was poor correlation between the ELISA results and those of the other tests. Only the results of a direct immunobead test done on semen correlated fairly well with results from ELISAs done on serum. Further studies are needed on fertility-specific antigens that can be identified and isolated and then studied by means of ELISAs.
用于检测精子抗体的酶联免疫吸附测定（ELISA）可作为其他检测的有益补充。与其他一些检测相比，ELISA能提供更定量和详细的信息，但结果应与其他检测的结果进行比较。本研究旨在将以精子膜提取物为抗原的ELISA结果与其他检测结果进行关联。对34名精子抗体阳性和36名精子抗体阴性男性的精液、精浆和血清进行了检测。ELISA结果与其他检测结果之间的相关性较差。仅精液直接免疫珠试验的结果与血清ELISA结果有较好的相关性。需要对可识别、分离并通过ELISA进行研究的生育特异性抗原进行进一步研究。
