Durg Sharanbasappa, Veerapur Veeresh P, Thippeswamy B S, Ahamed Syed Mansoor
Department of Pharmacology, Sree Siddaganga College of Pharmacy, Tumkur, Karnataka, India.
Department of Quality Assurance, Sree Siddaganga College of Pharmacy, Tumkur, Karnataka, India.
Anc Sci Life. 2015 Oct-Dec;35(2):110-7. doi: 10.4103/0257-7941.171675.
Śilājatu (Shilajit; SJ) is claimed in traditional Indian medical practice to be useful in the treatment of nervous disorders, epilepsy and as antistress.
To investigate whether SJ possesses antiepileptic and antipsychotic activities in rodents.
Isonicotinyl hydrazine (INH), pentylenetetrazole (PTZ), apomorphine, phenytoin, diazepam, haloperidol and other chemicals of analytical grade were procured from standard companies. The antiepileptic activity of SJ was assessed using maximal electro shock (MES)-induced seizures in rats, INH and PTZ-induced seizures in mice. The antipsychotic effect of SJ was evaluated using apomorphine-induced climbing and stereotyped behaviours respectively, in mice and rats.
SJ (25 and 50 mg/kg, p.o.) was given orally once daily for 15 days in all the rodent models. On the test day, SJ was administered 1 h prior to electric shock or chemical inducers (INH/PTZ/apomorphine) in experimental animals; the animals were then observed for different phases of seizures and psychotic behaviours. In addition, gamma-aminobutyric acid (GABA) content in the brain of rats and mice was estimated in seizure models.
The data were expressed as mean ± standard error of mean. Statistical comparisons were performed by one-way ANOVA followed by Tukey's post-test using Graph Pad Prism version 5.0, USA. A P < 0.05 was considered significant.
SJ pretreatment significantly inhibited the seizures induced by MES, INH and PTZ in a dose dependent manner. Further, SJ augmented brain GABA levels to normal, decreased by INH and PTZ in mice brain. SJ pretreatment also significantly inhibited the climbing and stereotyped behaviours induced by apomorphine. The present data seems to confirm the antiepileptic activity of SJ which may be because of enhancing the GABAergic system. The antipsychotic activity observed may be due to anti-dopaminergic and/or GABA-mimetic actions.
在传统印度医学实践中，希拉季特（Shilajit；SJ）据称可用于治疗神经紊乱、癫痫以及具有抗应激作用。
研究SJ在啮齿动物中是否具有抗癫痫和抗精神病活性。
异烟酰肼（INH）、戊四氮（PTZ）、阿扑吗啡、苯妥英、地西泮、氟哌啶醇及其他分析纯化学试剂购自标准公司。使用大鼠最大电休克（MES）诱导的癫痫发作、小鼠INH和PTZ诱导的癫痫发作来评估SJ的抗癫痫活性。分别使用阿扑吗啡诱导的小鼠和大鼠攀爬及刻板行为来评估SJ的抗精神病作用。
在所有啮齿动物模型中，SJ（25和50mg/kg，口服）每日口服一次，持续15天。在测试日，在实验动物中，于电击或化学诱导剂（INH/PTZ/阿扑吗啡）前1小时给予SJ；然后观察动物癫痫发作和精神病行为的不同阶段。此外，在癫痫模型中估计大鼠和小鼠脑中γ-氨基丁酸（GABA）的含量。
数据以平均值±平均标准误差表示。使用美国Graph Pad Prism 5.0版软件进行单因素方差分析，随后进行Tukey事后检验进行统计比较。P<0.05被认为具有统计学意义。
SJ预处理以剂量依赖性方式显著抑制MES、INH和PTZ诱导的癫痫发作。此外，SJ使小鼠脑中被INH和PTZ降低的脑GABA水平恢复正常。SJ预处理还显著抑制阿扑吗啡诱导的攀爬和刻板行为。目前的数据似乎证实了SJ的抗癫痫活性，这可能是由于增强了GABA能系统。观察到的抗精神病活性可能归因于抗多巴胺能和/或GABA模拟作用。
