Ogino S, Bessho K, Harada T, Irifune M, Matsunaga T
Department of Otolaryngology, Osaka University Medical School, Japan.
Rhinology. 1993 Mar;31(1):27-31.
Multiple-antigen simultaneous test (MAST) is a new system for detecting allergen-specific IgE antibodies. Multiple antigens can be examined simultaneously in a short period of time by this method. The purpose of this study is to evaluate the usefulness of this method and to compare the results of MAST with those of RAST and intradermal skin test using 133 serum samples obtained from patients with nasal allergy. The positive rates of the main allergens detected by the MAST system are 56% for Japanese cedar, 31 for Dermatophagoides farinae (DF), 30% for Dermatophagoides pteronyssinus (DP), 27% for house dust (HD), and 27% for timothy grass. The positive rates of food allergens are very low. An average of 3.5 different allergens can be simultaneously detected in one serum. We have compared MAST and RAST with respect to nine allergens: HD, DF, cat, Japanese cedar, timothy, bahia, sweet vernal, velvet, and ragweed. There are statistically significant correlations between MAST and RAST for all allergens except ragweed, the correlation coefficients in the eight allergens are greater than r = 0.60, and total agreements exceed over 70%. Similarly, there also is a good correlation between MAST and skin test for the allergens: HD, cat, Japanese cedar, timothy grass, and ragweed. These results indicate the clinical usefulness of the MAST system for detecting specific IgE antibodies in patients with nasal allergy.
多抗原同步检测（MAST）是一种检测变应原特异性IgE抗体的新系统。通过该方法可在短时间内同时检测多种抗原。本研究旨在评估该方法的实用性，并将MAST的检测结果与放射变应原吸附试验（RAST）以及皮内皮肤试验的结果进行比较，研究使用了从鼻过敏患者中获取的133份血清样本。MAST系统检测出的主要变应原阳性率分别为：日本柳杉56%，粉尘螨（DF）31%，屋尘螨（DP）30%，屋尘（HD）27%，梯牧草27%。食物变应原的阳性率非常低。一份血清平均可同时检测出3.5种不同的变应原。我们比较了MAST和RAST对9种变应原的检测结果：HD、DF、猫、日本柳杉、梯牧草、百喜草、春黄菊、绒毛草和豚草。除豚草外，MAST和RAST对所有变应原的检测结果均具有统计学意义上的显著相关性，8种变应原的相关系数大于r = 0.60，总一致性超过70%。同样，MAST与HD、猫、日本柳杉、梯牧草和豚草等变应原的皮肤试验结果也具有良好的相关性。这些结果表明MAST系统在检测鼻过敏患者特异性IgE抗体方面具有临床实用性。
