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印度猪旋毛虫病的流行病学研究()

Epidemiological studies on trichinellosis in pigs () in India.

作者信息

Konwar Pranita, Singh Balbir Bagicha, Gill Jatinder Paul Singh

机构信息

School of Public Health and Zoonoses, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004 India.

出版信息

J Parasit Dis. 2017 Jun;41(2):487-490. doi: 10.1007/s12639-016-0834-x. Epub 2016 Sep 13.

Abstract

The present study was conducted to determine the prevalence of trichinellosis in domestic pigs ( in India. A total of 319 tissue and 279 serum samples were collected from 279 slaughter pigs. The samples were collected from small slaughter shops located in Assam, Chandigarh (Union territory), Punjab and Uttarakhand states of the country. The presence of spp. antibodies in serum was determined using a commercially available enzyme-linked immunosorbent assay (ELISA) kit and tissue samples were digested using the OIE recommended pepsin-HCl digestion method. The overall seroprevalence of trichinellosis was found to be 2.87 %. The age wise seroprevalence was found to be 2.83 % in pigs aged more than 6 months and 3.12 % in pigs <6 months of age. The seroprevalence was more in male (3.38 %) than female (1.96 %) pigs. None of the samples were found positive using pepsin-HCl digestion method. Since none of the samples was found positive for PCR assay could not be employed in the current study. The age, sex and management practices of the animals were not significantly ( > 0.05) associated with seroprevalence. The current research confirms that trichinellosis remains a rare zoonosis in India. However, extensive studies must be carried out in wildlife to identify reservoir hosts in the country.

摘要

本研究旨在确定印度家猪旋毛虫病的流行情况。从279头屠宰猪身上共采集了319份组织样本和279份血清样本。这些样本采自该国阿萨姆邦、昌迪加尔(联邦属地)、旁遮普邦和北阿坎德邦的小型屠宰场。使用市售酶联免疫吸附测定(ELISA)试剂盒测定血清中旋毛虫属抗体的存在情况,组织样本采用世界动物卫生组织推荐的胃蛋白酶 - 盐酸消化法进行消化。旋毛虫病的总体血清阳性率为2.87%。6个月以上猪的血清阳性率为2.83%,6个月以下猪的血清阳性率为3.12%。雄性猪(3.38%)的血清阳性率高于雌性猪(1.96%)。使用胃蛋白酶 - 盐酸消化法未发现任何样本呈阳性。由于未发现任何样本的PCR检测呈阳性,因此本研究未采用PCR检测。动物的年龄、性别和管理方式与血清阳性率无显著关联(P>0.05)。当前研究证实,旋毛虫病在印度仍然是一种罕见的人畜共患病。然而,必须对野生动物进行广泛研究,以确定该国的储存宿主。

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