Strzelecka Dominika, Holman Stephen W, Eyers Claire E
Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Zwirki i Wigury 93, 02-089 Warsaw, Poland; Centre for Proteome Research, Department of Biochemistry, Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK.
Centre for Proteome Research, Department of Biochemistry, Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK.
Int J Mass Spectrom. 2015 Nov 30;391:157-160. doi: 10.1016/j.ijms.2015.07.004.
Dimethyl sulfoxide (DMSO) has been advocated as a beneficial additive to electrospray solvents for peptide analysis due to the improved ionisation efficiency conferred. Previous reports have shown that the resultant improvements in peptide ion signal intensities are non-uniform. As a result, it was hypothesised that inclusion of DMSO in electrospray solvents could be detrimental to the outcome of intensity-based label-free absolute quantification approaches, specifically the top 3 method. The effect of DMSO as a mobile phase additive in top 3 label-free quantification was therefore evaluated. We show that inclusion of DMSO enhances data quality, improving the precision and number of proteins quantified, with no significant change to the quantification values observed in its absence.
二甲基亚砜（DMSO）已被提倡作为肽分析电喷雾溶剂的有益添加剂，因为它能提高电离效率。先前的报告表明，由此导致的肽离子信号强度的提高并不均匀。因此，有人推测在电喷雾溶剂中加入DMSO可能不利于基于强度的无标记绝对定量方法的结果，特别是前3种方法。因此，评估了DMSO作为流动相添加剂在前3种无标记定量中的作用。我们表明，加入DMSO可提高数据质量，提高定量蛋白质的精度和数量，而在不加入DMSO的情况下观察到的定量值没有显著变化。
