Chair of Proteomics and Bioanalytics, Center of Life and Food Sciences Weihenstephan, Technische Universität München, Freising, Germany.
Nat Methods. 2013 Oct;10(10):989-91. doi: 10.1038/nmeth.2610. Epub 2013 Aug 25.
We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can be easily implemented on any LC-MS/MS system without modification to hardware or software and at no additional cost.
我们报告称，在液相色谱溶剂中低比例的二甲基亚砜（DMSO）会强烈增强肽的电喷雾电离，从而将基于自上而下的蛋白质组学中蛋白质鉴定的灵敏度提高多达十倍。该方法无需对硬件或软件进行修改，也无需额外花费，即可轻松在任何 LC-MS/MS 系统上实现。
