Yang Qing-Yuan, Sun Zhe, Tan Fei-Fei, Guo Ling-Hua, Wang Yu-Zhou, Wang Juan, Wang Zhi-Yan, Wang Li-Lin, Li Xiang-Dong, Xiao Yan, Tian Ke-Gong
Qing-Yuan Yang, Zhe Sun, Fei-Fei Tan, Ling-Hua Guo, Yu-Zhou Wang, Juan Wang, Zhi-Yan Wang, Li-Lin Wang, Xiang-Dong Li, Yan Xiao, Ke-Gong Tian, National Research Center for Veterinary Medicine, Luoyang 471003, Henan Province, China.
World J Virol. 2016 Feb 12;5(1):23-30. doi: 10.5501/wjv.v5.i1.23.
To test the pathogenicity of pseudorabies virus (PRV) variant HN1201 and compare its pathogenicity with a classical PRV Fa strain.
The pathogenicity of the newly-emerging PRV variant HN1201 was evaluated by different inoculating routes, virus loads, and ages of pigs. The classical PRV Fa strain was then used to compare with HN1201 to determine pathogenicity. Clinical symptoms after virus infection were recorded daily and average daily body weight was used to measure the growth performance of pigs. At necropsy, gross pathology and histopathology were used to evaluate the severity of tissue damage caused by virus infection.
The results showed that the efficient infection method of RPV HN1201 was via intranasal inoculation at 10(7) TCID50, and that the virus has high pathogenicity to 35- to 127-d old pigs. Compared with Fa strain, pigs infected with HN1201 showed more severe clinical symptoms and pathological lesions. Immunochemistry results revealed HN1201 had more abundant antigen distribution in extensive organs.
All of the above results suggest that PRV variant HN1201 was more pathogenic to pigs than the classical Fa strain.
检测伪狂犬病病毒（PRV）变异株HN1201的致病性，并将其致病性与经典PRV Fa株进行比较。
通过不同接种途径、病毒载量和猪的年龄评估新出现的PRV变异株HN1201的致病性。然后用经典PRV Fa株与HN1201进行比较以确定致病性。每天记录病毒感染后的临床症状，并用平均日体重衡量猪的生长性能。尸检时，采用大体病理学和组织病理学评估病毒感染引起的组织损伤严重程度。
结果表明，RPV HN1201的有效感染方法是经鼻接种10(7) TCID50，该病毒对35至127日龄的猪具有高致病性。与Fa株相比，感染HN1201的猪表现出更严重的临床症状和病理病变。免疫组化结果显示HN1201在广泛器官中的抗原分布更丰富。
上述所有结果表明，PRV变异株HN1201对猪的致病性高于经典Fa株。
