Lian Zhengmin, Liu Panrao, Zhu Zhenbang, Sun Zhe, Yu Xiuling, Deng Junhua, Li Ruichao, Li Xiangdong, Tian Kegong
Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
Luoyang Putai Biotech Co., Ltd., Luoyang 471003, China.
Viruses. 2023 Sep 20;15(9):1966. doi: 10.3390/v15091966.
Pseudorabies virus (PRV) variants were discovered in immunized pigs in Northern China and have become the dominant strains since 2011, which caused huge economic losses. In this study, a classical PRV strain was successfully isolated in a PRV gE positive swine farm. The complete genome sequence was obtained using a high-throughput sequencing method and the virus was named JS-2020. The nucleotide homology analysis and phylogenetic tree based on complete genome sequences or gC gene showed that the JS-2020 strain was relatively close to the classical Ea strain in genotype II clade. However, a large number of amino acid variations occurred in the JS-2020 strain compared with the Ea strain, including multiple immunogenic and virulence-related genes. In particular, the gE protein of JS-2020 was similar to earlier Chinese PRV strains without Aspartate insertion. However, the amino acid variations analysis based on major immunogenic and virulence-related genes showed that the JS-2020 strain was not only homologous with earlier PRV strains, but also with strains isolated in recent years. Moreover, the JS-2020 strain was identified as a recombinant between the GXGG-2016 and HLJ-2013 strains. The pathogenicity analysis proved that the PRV JS-2020 strain has typical neurogenic infections and a strong pathogenicity in mice. Together, a novel recombinant classical strain was isolated and characterized in the context of the PRV variant pandemic in China. This study provided some valuable information for the study of the evolution of PRV in China.
在中国北方的免疫猪群中发现了伪狂犬病病毒（PRV）变异株，自2011年以来已成为优势毒株，造成了巨大的经济损失。在本研究中，在一个PRV gE阳性猪场成功分离出一株经典PRV毒株。采用高通量测序方法获得了其全基因组序列，该病毒被命名为JS-2020。基于全基因组序列或gC基因的核苷酸同源性分析和系统发育树显示，JS-2020毒株在基因型II分支中与经典Ea毒株相对接近。然而，与Ea毒株相比，JS-2020毒株发生了大量氨基酸变异，包括多个免疫原性和毒力相关基因。特别是，JS-2020的gE蛋白与早期中国PRV毒株相似，没有天冬氨酸插入。然而，基于主要免疫原性和毒力相关基因的氨基酸变异分析表明，JS-2020毒株不仅与早期PRV毒株同源，也与近年来分离的毒株同源。此外，JS-2020毒株被鉴定为GXGG-2016和HLJ-2013毒株之间的重组体。致病性分析证明，PRV JS-2020毒株在小鼠中具有典型的神经源性感染和较强的致病性。总之，在中国PRV变异株大流行的背景下，分离并鉴定了一株新型重组经典毒株。本研究为中国PRV进化研究提供了一些有价值的信息。
