Department of Physics, Chemistry and Biology, Linköping University , SE-581 83, Linköping, Sweden.
Department of Science and Technology, Linköping University , SE-601 74, Norrköping, Sweden.
J Am Chem Soc. 2016 Mar 2;138(8):2504-7. doi: 10.1021/jacs.5b13149. Epub 2016 Feb 19.
Single molecule enzymology provides an opportunity to examine details of enzyme mechanisms that are not distinguishable in biomolecule ensemble studies. Here we report, for the first time, detection of the current produced in an electrocatalytic reaction by a single redox enzyme molecule when it collides with an ultramicroelectrode. The catalytic process provides amplification of the current from electron-transfer events at the catalyst leading to a measurable current. This new methodology monitors turnover of a single enzyme molecule. The methodology might complement existing single molecule techniques, giving further insights into enzymatic mechanisms and filling the gap between fundamental understanding of biocatalytic processes and their potential for bioenergy production.
单分子酶学为研究生物分子整体研究中无法区分的酶机制细节提供了机会。在这里，我们首次报道了在单个氧化还原酶分子与超微电极碰撞时，通过电催化反应检测到的电流。该催化过程放大了催化剂中电子转移事件产生的电流，从而产生可测量的电流。这种新方法监测单个酶分子的周转率。该方法可能补充现有的单分子技术，进一步深入了解酶机制，并填补生物催化过程基本理解与其在生物能源生产中的潜力之间的差距。
